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Total Triiodothyronine (T3) ELISA for serum or plasma 0.1-10 ng/ml Dialab
Product code: Z01208
Pack Size: 96 Wells
CE Verified: CE IVD
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
Thyroid function test panels typically include measurement of triiodothyronine (T3) and Thyroxine (T4), the thyroid hormones that control and regulate metabolism. In the blood most of the thyroid hormone is bound to protein but there is also a free, unbound fraction that is biologically active. Measurement of serum T3 concentration is generally regarded as a valuable tool in the diagnosis of thyroid dysfunction and is used to help diagnose hyperthyroidism.
Normal 0.49-2.02 ng/ml
This assay is intended for the quantitative determination of total T3 in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. T3 in the sample competes with horseradish peroxidase (HRP) labelled T3 for binding onto the limited number of anti- T3 sites coated on the microplate. After incubation, the bound/free separation is performed by a simple- solid-phase washing. The enzyme in the bound-fraction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of T3 in the sample.
Antibody coated Microtiter wells (Sheep Anti-T3 monoclonal): 12x8 well strips
Calibrator set (0.0, 0.5, 1.0, 2.5, 5.0, 10.0 ng/ml x 1.0ml)
Enzyme Conjugate (T3-HRP): 1x6ml
Sample diluent (buffer and binding protein inhibitors): 1x6ml
TMB Substrate Solution : 1x12ml
Stop Solution: 1x12ml
Wash Buffer 40X: 1x25ml
Sample volume: 50µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (30 min, 30min, 10 min in the dark. All at room temperature)
Calibration range: 0 – 10.0 ng/ml
Sensitivity: 0.1 ng/ml
Store at 2-8°C.
Normal 0.49-2.02 ng/ml
This assay is intended for the quantitative determination of total T3 in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. T3 in the sample competes with horseradish peroxidase (HRP) labelled T3 for binding onto the limited number of anti- T3 sites coated on the microplate. After incubation, the bound/free separation is performed by a simple- solid-phase washing. The enzyme in the bound-fraction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of T3 in the sample.
Antibody coated Microtiter wells (Sheep Anti-T3 monoclonal): 12x8 well strips
Calibrator set (0.0, 0.5, 1.0, 2.5, 5.0, 10.0 ng/ml x 1.0ml)
Enzyme Conjugate (T3-HRP): 1x6ml
Sample diluent (buffer and binding protein inhibitors): 1x6ml
TMB Substrate Solution : 1x12ml
Stop Solution: 1x12ml
Wash Buffer 40X: 1x25ml
Sample volume: 50µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (30 min, 30min, 10 min in the dark. All at room temperature)
Calibration range: 0 – 10.0 ng/ml
Sensitivity: 0.1 ng/ml
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life NULL
- Sensitivity 0.1ng/ml
- Application This assay is intended for the quantitative determination of Total T3 in human serum or plasma for diagnostic use.
- Specificity NULL
- Contents Antibody coated Microtiter wells (Sheep Anti-T3 monoclonal): 12x8 well strips
Calibrator set (0.0, 0.5, 1.0, 2.5, 5.0, 10.0 ng/ml x 1.0ml)
Enzyme Conjugate (T3-HRP): 1x6ml
Sample diluent (buffer and binding protein inhibitors): 1x6ml
TMB Substrate Solution : 1x12ml
Stop Solution: 1x12ml
Wash Buffer 40X: 1x25ml - Time Required Incubation time: 70 minutes (30 min, 30min, 10 min in the dark. All at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition Ambient
- Storage Condition Fridge
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00