Total Bile Acids TBA assay 1-180̲mol/L liquid chemistry serum or plasma colorimetric kinetic method enzymatic recycling Dialab 4x22.5ml,1x30ml

Total Bile Acids kit enzymatic recycling method colorimetric kinetic assay manufactured by Dialab. Reagent 1: 4x22.5ml Reagent 2: 1x30ml.

Total bile acids are metabolised in the liver and hence serve as a marker of liver function. Obstetric Cholestasis is the most common liver disease during pregnancy and is linked to foetal distress, premature labour and still birth. Raised levels of Bile Acids in pregnancy are indicative of obstetric cholestasis and increased bile acids in fasting or postprandial state is considered a specific indicator of liver disease. Serum total bile acids concentrations may be increased in patients with acute hepatitis, chronic hepatitis, liver sclerosis and liver cancer.

Decreases in bile acids can also be of clinical interest and are associated with bile acid malabsorption.

The Dialab dual liquid reagent system can be used to measure Bile Acids in serum or EDTA plasma with a measuring range of 1-180µmol/L. 3-alpha-hydroxysteroid dehydrogenase (3-alpha-HSD) oxidizes bile acids to 3-keto steroids, whereby Thio-NAD is reduced to Thio-NADH. The reaction is reversible, and the same enzyme can convert 3-keto steroids and NADH to bile acids and NAD (recycling of bile acids). The rate of formation for Thio-NADH is determined by measuring the change of absorbance at 405nm.

This Bile Acids kit contains 4x22.5ml substrate and 1x30ml enzyme reagent.

Bile Acids reagents have an open vial stability of 30 days when stored tightly capped at 2-8C.

Use with the Dialab Bile Acids Standard (903210).

Also available is the Sentinel Diagnostics Bile Acid Control Set (1600201)- if you are looking for a third party QC.

Suitable for use in manual or automated methods - applications for a range of analysers are available.