Cholesterol reagent for quantitative in vitro determination in human serum or plasma 0.08 – 19.4 mmol/L CHOD-PAP method Dialab

Cholesterol reagent for quantitative in vitro determination in human serum or plasma on photometric systems 0.08 – 19.4 mmol/L CHOD-PAP method Dialab

Cholesterol is a component of cell membranes and a precursor for steroid hormones and bile acids synthesised by the body and absorbed with food. Cholesterol is transported in plasma via lipoproteins, namely complexes between lipids and apolipoproteins. There are four classes of lipoproteins: high density lipoproteins (HDL), low density lipoproteins (LDL), very low density lipoproteins (VLDL) and chylomicrons. While LDL is involved in the cholesterol transport to the peripheral cells, HDL is responsible for the cholesterol uptake from the cells. The four different lipoprotein classes show distinct relationship to coronary atherosclerosis. LDLcholesterol (LDL-C) contributes to atherosclerotic plaque formation within the arterial intima and is strongly associated with coronary heart disease (CHD) and related mortality. Even with total cholesterol within the normal range an increased concentration of LDL-C indicates high risk. HDL-C has a protective effect impeding plaque formation and shows an inverse relationship to CHD prevalence. In fact, low HDL-C values constitute an independent risk factor. The determination of the individual total cholesterol (TC) level is used for screening purposes while for a better risk assessment it is necessary to measure additionally HDL-C and LDL-C.

This assay is a colorimetric, Trinder based method to determine concentrations of cholesterol after enzymatic hydrolysis and oxidation. Cholesterol ester and water are enzymatically converted by cholesterol esterase to produce cholesterol and fatty acid. Cholesterol and oxygen are then converted to cholesterol-3-one and hydrogen peroxide by the enzyme cholesterol oxidase. The resultant hydrogen peroxide reacts with Phenol and 4 Aminoantipyrine in the presence of peroxidase to generate the colorimetric indicator quinoneimine. The intensity of the pink/red colour is proportional to the cholesterol concentration in the sample.

The assay should be calibrated using the Cholesterol Standard (D95114) or Diacal Auto (D98485SV) and suggested control material is the Diacon controls (D98481SV and D98482SV).

Store at 2-8°C