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ELISA for IgG and IgM and mixed conjugate for antibodies against gangliosides for GM1 GD1 and GQ1b 12 profiles BÜHLMANN GanglioComb™-Light
Product code: EK-GCL-S
Pack Size: 96 wells
CE Verified: CE IVD
Product Information
The BÜHLMANN GanglioCombi™ Light (EK-GCL-S) ELISA screens with IgG/ IgM mix conjugate and quantifies with IgG and IgM conjugates for autoantibodies against the gangliosides GM1, GD1b and GQ1b in human serum for differentiation of particular neuropathies
BÜHLMANN GanglioCombi™ Light is a reduced profile of the BÜHLMANN GangliCombi™ MAG ELISA for a targeted screening of antibodies against the most frequent gangliosides (GM1, GD1b and GQ1b). It includes the same calibrator and controls as the BÜHLMANN GangliCombi™ MAG ELISA. Patients may be analysed for antibodies against each of these gangliosides so to obtain an IgG and IgM “mini-profile” per patient.
Anti-GM1 antibodies: anti-GM1 antibodies (IgG) are implicated in the pathogenesis of Guillain-Barré Syndrome (GBS) and anti-GM1 antibodies (IgM) in multifocal motor neuropathy (MMN) and motor neuron diseases (MND). anti-GM1 antibodies (IgM) may be present as monoclonal IgM paraprotein or as a polyclonal IgM. Polyclonal IgG may be detected in patients with motor neuropathies. High GM1 antibody titers may be detected in association with GBS following Campylobacter jejuni infection. The fact that GM1 is the only common Glycolipid antigen reactive with all of the GM1 antibodies found in motor nerve disorders suggests that it is the relevant target antigen in neural tissue.
Anti-GD1b antibodies: Reactivity against gangliosides containing disialosyl groups, particularly GD1b, has been reported in isolated cases of acute and chronic idiopathic ataxic neuropathies.
The clinical picture comprises a chronic neuropathy with marked sensory ataxia and areflexia and with relatively preserved motor function in the limbs. In addition many patients have preserved motor function. When present in their entirety, these clinical features are described under the acronym CANOMAD (chronic, ataxic, neuropathy, opthalmoplegia, IgM paraprotein, cold agglutinins, disialosy antibodies).
Anti-GQ1b antibodies: Anti- IgG GQ1b antibodies are closely associated with Miller-Fisher Syndrome (MFS), which is discussed to be a variant of Guillain-Barré Syndrome. Up to 100% of MFS patients have these antibodies. Anti-GQ1b antibodies decay rapidly with clinical recovery and usually are not found in normal and disease control samples (non-immune-mediated neuropathies). The close serologic-clinical association make anti-GQ1b antibodies in suspected cases of overlapping symdroms in neuropathies a useful diagnostic marker for physicians with therapeutic significance.
Product Details:
Specimen Type: Serum
Sample volume: 30µl
Isotype: IgG and IgM separate and IgG/IgM mix conjugate
Test type: ELISA
Note: Cold test conditions required
Time to Result: 5 hour
CE/IVD marked: Yes
Number of tests: 12 profiles
Storage conditions: 2-8C Do not freeze the calibrators or controls
Interpretative criteria:
Strongly positive: >100%
Positive: 50 - 100%
Cut-off : 50
Greyzone: 30-50%
Negative: < 30%
BÜHLMANN GanglioCombi™ Light is a reduced profile of the BÜHLMANN GangliCombi™ MAG ELISA for a targeted screening of antibodies against the most frequent gangliosides (GM1, GD1b and GQ1b). It includes the same calibrator and controls as the BÜHLMANN GangliCombi™ MAG ELISA. Patients may be analysed for antibodies against each of these gangliosides so to obtain an IgG and IgM “mini-profile” per patient.
Anti-GM1 antibodies: anti-GM1 antibodies (IgG) are implicated in the pathogenesis of Guillain-Barré Syndrome (GBS) and anti-GM1 antibodies (IgM) in multifocal motor neuropathy (MMN) and motor neuron diseases (MND). anti-GM1 antibodies (IgM) may be present as monoclonal IgM paraprotein or as a polyclonal IgM. Polyclonal IgG may be detected in patients with motor neuropathies. High GM1 antibody titers may be detected in association with GBS following Campylobacter jejuni infection. The fact that GM1 is the only common Glycolipid antigen reactive with all of the GM1 antibodies found in motor nerve disorders suggests that it is the relevant target antigen in neural tissue.
Anti-GD1b antibodies: Reactivity against gangliosides containing disialosyl groups, particularly GD1b, has been reported in isolated cases of acute and chronic idiopathic ataxic neuropathies.
The clinical picture comprises a chronic neuropathy with marked sensory ataxia and areflexia and with relatively preserved motor function in the limbs. In addition many patients have preserved motor function. When present in their entirety, these clinical features are described under the acronym CANOMAD (chronic, ataxic, neuropathy, opthalmoplegia, IgM paraprotein, cold agglutinins, disialosy antibodies).
Anti-GQ1b antibodies: Anti- IgG GQ1b antibodies are closely associated with Miller-Fisher Syndrome (MFS), which is discussed to be a variant of Guillain-Barré Syndrome. Up to 100% of MFS patients have these antibodies. Anti-GQ1b antibodies decay rapidly with clinical recovery and usually are not found in normal and disease control samples (non-immune-mediated neuropathies). The close serologic-clinical association make anti-GQ1b antibodies in suspected cases of overlapping symdroms in neuropathies a useful diagnostic marker for physicians with therapeutic significance.
Product Details:
Specimen Type: Serum
Sample volume: 30µl
Isotype: IgG and IgM separate and IgG/IgM mix conjugate
Test type: ELISA
Note: Cold test conditions required
Time to Result: 5 hour
CE/IVD marked: Yes
Number of tests: 12 profiles
Storage conditions: 2-8C Do not freeze the calibrators or controls
Interpretative criteria:
Strongly positive: >100%
Positive: 50 - 100%
Cut-off : 50
Greyzone: 30-50%
Negative: < 30%
Specification
- Brand BÜHLMANN
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life NULL
- Sensitivity NULL
- Application Screening for autoantibodies, Anti-Gangliosides GM1,GD1b, GQ1b. Specific IgG and IgM conjugates, and IgG/IgM mix conjugate is also supplied for quantification and confirmation. For diagnostic use.
- Specificity NULL
- Contents 12 x 8 wells, 3x plate seals, 1x100ml Wash Buffer (10X), 1x100ml Incubation buffer, 1 x Calibrator (lyophilised) 1.5ml, Controls 3 levels (lyophilised) 1.5ml; 1x11ml Mixed IgG/IgM conjugate; 1x11ml IgG conjugate, 1x11ml IgM conjugate, 1x11ml TMB substrate, 1x11ml stop solution.
- Time Required Incubation time 4.5h (2h at 2-8°C, 2h at 2-8°C, 30min at 18-28°C)
Storage Details
- Pack Description 96 wells
- Shipping Condition Ambient
- Storage Condition Fridge
- Pack Length (m) 0.22
- Pack Width (m) 0.16
- Pack Height (m) 0.11
- Pack Weight (kg) 0.40