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17-beta-Estradiol ELISA for serum or plasma 20-2000 pg/ml Dialab
Product code: K00194
Pack Size: 96 Wells
CE Verified: CE IVD
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
17-beta-oestradiol (17-β-Estradiol) is the major form of oestrogen in humans, with critical impact of reproductive function and other organs including bone structure. During the reproductive years most oestradiol is women is produced by the ovaries, with smaller amounts also produced by the adrenal cortex. In men the testes produce oestradiol. Plasma oestradiol is largely bound to sex hormone binding globulin or albumin, only a faction is free and biologically active. Serum oestradiol measurement in women reflects primarily the activity of the ovaries and is used to help evaluate fertility and menstrual irregularities and monitor ovarian follicular function. Oestradiol is also involved in male fertility.
Normal levels:
Children <60 pg/ml
Men <40 pg/ml
Women Follicular phase: 30-100 pg/ml
Ovulatory peak: 130-350 pg/ml
Lutenic phase: 50-180 pg/ml
Menopause <60 pg/ml
This assay is intended for the quantitative determination of 17-β-Estradiol in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. 17-β-Estradiol in the sample competes with 17-β-Estradiol-HRP for binding onto the limited number of anti-17-β-Estradiol antibodies coated on the microplate. After incubation, the bound/free separation is performed by a simple solid-phase washing step. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of 17-β-Estradiol in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-17-β-Estradiol IgG): 12x8 well strips
Calibrator set (0, 20, 120, 300, 600, 2000 pg/ml): 6x0.5ml
Control: 1x0.5ml
Enzyme Conjugate (17-β-Estradiol-HRP): 1x22ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml
Once open reagents can be kept at 2-8°C for up to 6 months
Sample volume: 25µl human serum or plasma to be run in duplicate
Incubation time: 150 minutes (120 min at 37°C, 30 min at room temperature)
Measurable range: 20-2000 pg/ml
Sensitivity: 8.68 pg/ml
Store at 2-8°C.
Normal levels:
Children <60 pg/ml
Men <40 pg/ml
Women Follicular phase: 30-100 pg/ml
Ovulatory peak: 130-350 pg/ml
Lutenic phase: 50-180 pg/ml
Menopause <60 pg/ml
This assay is intended for the quantitative determination of 17-β-Estradiol in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. 17-β-Estradiol in the sample competes with 17-β-Estradiol-HRP for binding onto the limited number of anti-17-β-Estradiol antibodies coated on the microplate. After incubation, the bound/free separation is performed by a simple solid-phase washing step. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of 17-β-Estradiol in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-17-β-Estradiol IgG): 12x8 well strips
Calibrator set (0, 20, 120, 300, 600, 2000 pg/ml): 6x0.5ml
Control: 1x0.5ml
Enzyme Conjugate (17-β-Estradiol-HRP): 1x22ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml
Once open reagents can be kept at 2-8°C for up to 6 months
Sample volume: 25µl human serum or plasma to be run in duplicate
Incubation time: 150 minutes (120 min at 37°C, 30 min at room temperature)
Measurable range: 20-2000 pg/ml
Sensitivity: 8.68 pg/ml
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life NULL
- Sensitivity 8.68 pg/ml
- Application This assay is intended for the quantitative determination of 17-β-Estradiol in human serum or plasma for diagnostic use.
- Specificity Specificity for Estradiol 100%, Estrone 2%, Estriol 0.39%, Testosterone 0.02%, Cortisol < 0.007%, Progesterone < 0.0003%, DHEA-S < 0.0001%
- Contents Antibody coated Microtiter wells (Anti-17-β-Estradiol IgG): 12x8 well strips,
Calibrator set (0, 20, 120, 300, 600, 2000 pg/ml): 6x0.5ml
Control: 1x0.5ml
Enzyme Conjugate (17-β-Estradiol-HRP): 1x22ml
Substrate Solution (H2O2-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml - Time Required Incubation time: 150 minutes (120 min at 37°C, 30 min at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition Ambient
- Storage Condition Fridge
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00