17-alpha-OH-Progesterone ELISA for serum or plasma 0.2-19.2 ng/ml Dialab

17-Hydroxyprogesterone (17-α-OH-Progesterone or 17-α-OHP) is a C-21 steroid hormone produced in the adrenal gland and gonads, during the synthesis of glucocorticoids and sex steroids. It is derived from progesterone via 17-hydroxylase, a P450c17 enzyme or from 17-.
Serum 17-α--OHP levels are age-dependent, with peak levels observed during foetal life and the immediate postnatal period. During the first week of life, serum 17-α-OHP levels fall ~50-fold as compared to cord blood values. A small transient increase occurs in male infants 30-60 days. Levels for both sexes remain at low during childhood but progressively increase during puberty reaching adult levels of ~100ng/dL (~3.03nmol/L). As with cortisol, serum 17-α-OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night. In addition, ovarian production of 17-α-OHP increases during the luteal phase of the menstrual cycle. 17-α-OHP is a natural progestin and increases in the third trimester of pregnancy primarily due to foetal adrenal production.
Normal levels
Children 3-90 ng/dL
Women prior to ovulation 15-70ng/dL
Women during luteal phase 35-290ng/dl
Measurements of 17-α--OHP levels are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydoxylase and 11-β-hydroxylase, lead to a build-up of 17-α-OHP. In contrast, the rare patient with 17-α-hydroxylase deficiency will have very low or undetectable levels of 17-α-OHP. Elevated serum 17-α-OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia.
This assay is intended for the quantitative determination of 17-α-OHP in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. 17-α-OHP in the sample competes with the antigenic 17-α-OH-Progesterone-HRP for binding onto the limited number of anti-17-α-OH-Progesterone antibodies coated on the microplate. After incubation, the bound/free separation is performed by a simple solid-phase washing step. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of 17-α-OH-Progesterone in the sample.

Kit contents:
Antibody coated Microtiter wells (Anti-17-α-OH-Progesterone IgG): 12x8 well strips
Calibrator set (0, 0.2, 0.4, 1.6, 6.4, 19.2 ng/ml): 6x1ml
Control: 1x1ml
Enzyme Conjugate (17-α-OH-Progesterone-HRP): 1x6ml
Substrate Solution (H₂O₂-TMB 0.26g/L): 1x15ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x15ml

Sample volume: 50µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (60 min at 37°C, 15 min at room temperature)
Measurable range: 0.2-19.2 ng/ml
Sensitivity: 0.09ng/ml
Store at 2-8°C.