ARM-D real-time PCR kit for detection of six plasmid-mediated ampC gene families MOX DHA ACC EBC FOX CMY-2100 reactions

AmpC β-lactamases are clinically important cephalosporinases that are resistant to most β-Lactam antibiotics. AmpC enzymes are chromosomally encoded in many bacterial species and can be inducible and overexpressed. Overexpression can lead to resistance to most β-lactam antibiotics. The occurrence of transmissible plasmids with acquired genes for AmpC β-lactamases often results in increased β-lactamase production, compared to chromosomally-expressed non-induced ampC genes. Additionally, plasmid-mediated AmpC β-lactamases can appear in organisms lacking or having low-level expression of a chromosomal ampC gene. Resistance due to plasmid-mediated AmpC enzymes can be broad in spectrum and often hard to detect. As such, it is clinically useful to detect and discriminate between plasmid-mediated and chromosomally expressed AmpC β-lactamases.
The Streck ARM-D Kit, ampC + Master Mix is a qualitative molecular test that allows for identification of six plasmid-mediated ampC gene families: MOX, DHA, ACC, EBC, FOX, and CMY-21. Additionally, an endogenous internal control (IC) that targets a conserved region common in Gram-negative bacteria is included to reduce false negatives due to PCR inhibition, DNA degradation, or poor extraction. Kit includes a premixed 2X master mix requiring a single pipetting step (Supermix: PhilisaFAST® DNA Polymerase, dNTPs, MgCl2 and reaction buffer).
This test utilises sequence-specific primer pairs for the PCR amplification of each family as well as fluorescently-labelled, target-specific DNA probes for detection by real-time PCR. The Streck ARM-D Kit, ampC can differentiate a plasmid-mediated ampC β-lactamase gene from a chromosomal ampC β-lactamase gene; provided the two genes are not from the same chromosomal origin. This test generates data in under one hour.
Streck ARM-D Kit, ampC has been validated using PhilisaFAST® DNA Polymerase Kit with the Applied Biosystems (ABI) QuantStudio 7 (QS7) Flex Real-Time System, ABI 7500 Fast Real-Time PCR System, Bio-Rad CFX96 Touch™ Real-Time PCR Detection System, QIAGEN Rotor-Gene® Q, and Streck Zulu RT™ PCR System.