Serum Calprotectin ELISA
BÜHLMANN sCAL
The sCAL ELISA is a microtitre based assay kit for determination of the dimeric format of calprotectin in serum samples.
BÜHLMANN sCAL ELISA
All reagents, standards and controls are provided in one kit and are in a ready to use format.
There is no requirement for reconstituting any component, apart from the wash buffer so this minimises any errors and ensures a long shelf life.
The ELISA can be performed manually or on a DS2 automated processor to reduce hands-on time in higher throughput environments.
Assay range: 0.42 – 24µg/ml
Assay time: 75 minutes
Sample type: Serum.
Note: measurement in plasma from heparinized or citrated blood samples is possible, but be aware that normal values are slightly affected by the sample type used.
References
Helping to Manage Juvenile Idiopathic Arthritis
Great Ormond Street Hospital User Report – Serum Calprotectin Monitoring
Hurnakova: Serum calprotectin discriminates subclinical disease activity from ultra-sound defined remission in patients with RA in clinical remission.
Shows the “potential ability of serum calprotectin to discriminate ultrasound-defined remission from subclinical disease activity in RA patients who have achieved clinical remission. Overall, this study suggests that calprotectin might represent a valuable serological biomarker for confirming deep remission in RA patients”
Park et al. MRP8/14 serum levels as diagnostic markers for systemic juvenile idiopathic arthritis in children
with prolonged fever.
MRP8/14 serum analyses have been validated as a helpful tool supporting the diagnosis of SJIA in febrile children.
“The best results were obtained for BUHLMANN MRP8/14 ELISA (sensitivity 79.4%, specificity 89.0%, AUC 0.907)”
Ma et al. Serum Calprotectin Can Differentiate Between Sustained Remission and Low Disease Activity States in Rheumatoid Arthritis. ACR Paediatric Rheumatology symposium
This study demonstrated the role of calprotectin in differentiating subtle low disease activity states. Serum calprotectin was significantly different between low disease activity and remission as defined using different remission criteria. Its utility was seen with point remission as well as longitudinally over 1 year.
La et al. Serum calprotectin (S100A8/A9): a promising biomarker in diagnosis and follow-up in different subgroups of juvenile idiopathic arthritis
This study confirms the interest of sCal as a potential marker of response to treatment or risk of flares. It also highlights its role as a useful diagnostic tool and marker of disease activity, with a greater specificity than with C-reactive protein.
Marushko et al, Evaluation of serum calprotectin (MRP-8/MRP-14) levels in patients with juvenile idiopathic arthritis
sCal levels should be used to monitor the subclinical inflammatory activity in patients with JIA.