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Achromopeptidase crude bacteriolytic enzyme peptidase cell wall lysis gram negative positive 1g Wako

Product code: 014-09661

Pack Size: 1g
CE Verified: Research Use Only
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Product Information

Achromopeptidase crude bacteriolytic enzyme 1g produced by Wako Pure Chemicals

Achromopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al., has a broad range of bacteriolytic activity, lysing the cell walls of many pathological Gram-positive organisms as well as a number of Gram-negative organisms. Both purified (015-09951) and crude (014-09661) forms are available.

The bacteriolytic activity of Achromopeptidase is more potent than albumin lysozyme and it bacteriolytic spectrum is wider. It can be used for preparation of protoplasts from actinomycetes etc.

Activity: 900-1500 units/mg
Lyophilised enzyme

One unit of enzyme is defined as that which when present in 1ml of substrate suspension of lyophilised Micrococcus lysodeikticus IF O 3333 in Tris-HCl buffer caused turbidity (absorbance at 600nm) to decrease at the rate of 0.001 per minute at pH 8.0 and 37C.

Optimum pH 7.5-8.5
Diluent: Lactose as necessary.
Optimum ion strength for enzyme action: 0.01 mol/L NaCl [0.01 mol/L Tris-HCl buffer (pH 8.0)]
Source: Bacteria. Originally the source of this product was indicated as Achromobacter lyticus based on physiological and morphological properties of the bacteria. It was subsequently confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter.
Store at 2-10C

Specification

  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Specific Activity NULL
  • Purity NULL
  • Concentration 900-1500 U/mg
  • EC Number NULL
  • Optimal Temperature 37C
  • Optimal pH 7.5-8.5
  • Source Species Originally indicated as Achromobacter lyticus but 16SrDNA sequence highly homologous to Lysobacter
  • Application bacteriolytic enzyme
  • CAS Number NULL
  • Unit Definition One unit of enzyme is defined as that which when present in 1ml of substrate suspension of lyophilised Micrococcus lysodeikticus IF O 3333 in Tris-HCl buffer causes turbidity to decrease at the rate of 0.001 per minute at 37C, pH 8.0

Storage Details

  • Pack Description 1g
  • Shipping Condition Blue Ice
  • Storage Condition Fridge
  • Pack Length (m) 0.1
  • Pack Width (m) 0.05
  • Pack Height (m) 0.12
  • Pack Weight (kg) 0.00

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