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Phos-tag™ Agarose AG-503 3.0ml phosphate tag phosphate-affinity purification column chromatography spin-column Nard Institute Manac Inc Wako

Product code: 308-93563

Pack Size: 3mL
CE Verified: Research Use Only
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.

Product Information

Phos-tag™ Agarose AG-503 3ml manufactured by Nard Institute and Manac Incorporated and distributed in association with Wako Pure Chemicals.

Phos-tagTM is a novel labelling system for analysis of phosphorylation. The Phos-Tag, developed by the Department of Functional Molecular Science at Hiroshima University, recognises the phosphate monoester group. Phos-Tag agarose is perfect for separation and purification of native phosphoproteins and phosphorylated peptides from biological samples at physiological pH. The buffer contains no reducing agent and no detergent.

Phos-tag agarose can be used to produce a phosphate affinity chromatography column to bind inorganic phosphate and various phosphate dianions bound to amino acids, sugars and lipids. The captured anionic compound dissociates from the Phos-tag at pH 3-4. The eluted phosphate rich fraction can then be subjected to further downstream analysis such as electrophoresis and western blotting. The method is based on immobilized metal affinity chromatography (IMAC) using a phosphate-binding tag molecule (a dinuclear zinc (II) complex) attached on a highly cross-linked agarose (Phos-tag Agarose). Phos-tag agarose can also be used for spin-column purification methods.

The Phos-tag Agarose is supplied as a 50% (v/v) suspension of Zn2+ phosphate binding gel (AMP2- binding site = 3-5µmol/mL gel) in 20mM Tris acetate pH 7.4 with 20% (v/v) 2-propanol.

Store at 4°C.

Research Use Only.


  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Gelation Temperature NULL
  • Melting Temperature NULL
  • Gel Strength NULL
  • Electroendosmosis NULL
  • Application Type phosphate affinity column chromatography purification

Storage Details

  • Pack Description 3mL
  • Shipping Condition Blue Ice
  • Storage Condition Fridge
  • Pack Length (m) 0.15
  • Pack Width (m) 0.15
  • Pack Height (m) 0.22
  • Pack Weight (kg) 0.00

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