Phenyl Isothiocyanate Solution amino acid sequencing 5x5ml Wako

Phenyl Isothiocyanate Solution produced by FUJIFILM Wako Chemical Corporation

Phenyl Isothiocyanate is used as a derivation agent for primary, secondary and tertiary amines, and is a reagent for amino acid sequence analysis by the Edman method and amino acid analysis by HPLC. Analysis of amino acid composition is an important analysis in various fields such as protein/peptide research, food analysis and diagnosis of metabolic disorders. The PTC amino acid analysis method involves labelling with phenyl isothiocyanate (PITC), which has long been used as the Edman reagent, and is superior to other prelabeling methods in that it reacts with secondary amines and the molecular extinction coefficient for the standard compound is similar among any amino acids. However, the analysis requires complex gradients.

Wako offer a complete system consisting of a Wakopak WS-PTC column and a dedicated eluent allowing for analysis in short periods under simple eluting conditions. This system is applicable to any general-purpose HPLC system with few alterations in eluting conditions, and enables high-sensitivity, highly reproducible PTC amino acid analysis in short periods.

It reacts with the N-terminal amino acid in amino acid sequence analysis by the Edman degradation method. The resultant PTC derivative is treated with TFA, enriched and cut, derivatised into PTH, and amino acids identified. It reacts with an amino group containing a secondary amine. It has a detection limit of 10 pmol, and the derivative is stable for 6 hours at room temperature and 3 days at 4 degrees C.

Contains an ampule encapsulating nitrogen. Transfer the content to a glass-stoppered test tube after opening the ampule, and after use displace the inside of the test tube with nitrogen gas and store at -20 degrees C

Phenyl Isothiocyanate Solution 5x5ml

Molecular Formula=C₆H₅N:CS

Molecular Weight 135.19

CAS 103-72-0

Store -20°C