Restriction Endonuclease Nsp V DNA Cleavage 5 x 300U Nippon Gene Wako

Restriction Endonuclease Nsp I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation

Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.

Nsp I 5 x 300U

Recognition sequence:
5 '... TT ▼ CG AA ...... 3'
3 '... AA GC ▲ TT ...... 5'

Source : Nostoc species (ATCC 29411)

Activity: 1 - 10 units / μl

Reaction Conditions: 37°C in 1x Buffer L

Storage buffer :
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol

Buffer L 10X:
100 mmol / l Tris-HCl (pH 7.9 at 25 ° C)
100 mmol / l MgCl₂
10 mmol / l DTT

Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.


Store at -20°C