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Restriction Endonuclease Nde II DNA Cleavage 5 x 300U Nippon Gene Wako
Product code: 313-01863
Pack Size: 300Ux5
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Nde II (Mbo I) produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Nde II (Mbo I) 5 x 300U
Recognition sequence:
5 '... ▼ GATC ...... 3'
3 '... CTAG ▲ ...... 5'
Source : Neisseria denitrificans (NRCC 31009)
Activity: 3 - 15 units / μl
Reaction Conditions: 37°C in 1x reaction buffer
Storage buffer:
50 mmol / l KCl
10 mmol/l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer:
150 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.5)
10 mmol/L MgCl2
1 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 65°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Nde II (Mbo I) 5 x 300U
Recognition sequence:
5 '... ▼ GATC ...... 3'
3 '... CTAG ▲ ...... 5'
Source : Neisseria denitrificans (NRCC 31009)
Activity: 3 - 15 units / μl
Reaction Conditions: 37°C in 1x reaction buffer
Storage buffer:
50 mmol / l KCl
10 mmol/l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer:
150 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.5)
10 mmol/L MgCl2
1 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 65°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Neisseria denitrificans (NRCC 31009)
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 3- 15 units / μl
- Concentration 300U
- Cut Site 5 '... ▼ GATC ...... 3'
3 '... CTAG ▲ ...... 5' - Heat Inactivation 30 min at 65°C < 5% activity
Storage Details
- Pack Description 300Ux5
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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