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Restriction Endonuclease EcoR I DNA Cleavage 5 x 1200U Nippon Gene Wako
Product code: 310-00114
Pack Size: 12000Ux5
CE Verification: Research Use Only
- List Price
- £813.20
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
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Product Information
Restriction Endonuclease EcoR I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
EcoR I 5 x 12000U
Recognition sequence:
5 '... G ▼ AATT C ...... 3'
3 '... C TTAA ▲ G ...... 5'
Source : Escherichia coli RY13
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
400 mmol / l NaCl
5 mmol / l Potassium phosphate (pH 7.4)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
0.15% Triton-X-100
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C) 100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 70°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
EcoR I 5 x 12000U
Recognition sequence:
5 '... G ▼ AATT C ...... 3'
3 '... C TTAA ▲ G ...... 5'
Source : Escherichia coli RY13
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
400 mmol / l NaCl
5 mmol / l Potassium phosphate (pH 7.4)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
0.15% Triton-X-100
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C) 100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 70°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Escherichia coli RY13
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 12000U
- Cut Site 5 '... G ▼ AATT C ...... 3'
3 '... C TTAA ▲ G ...... 5' - Heat Inactivation 30 min at 70°C < 5% activity
Storage Details
- Pack Description 12000Ux5
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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