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Restriction Endonuclease Dra I DNA Cleavage 10 x 3500U Nippon Gene Wako
Product code: 310-00876
Pack Size: 3500Ux10
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Dra I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Dra I 10 x 3500U
Recognition sequence:
5 '... TTT ▼ AAA ...... 3'
3 '... AAA ▲ TTT ...... 5'
Source : Deinococcus radiophilus (ATCC 27603)
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer M
Storage buffer:
50 mmol / l KCl
50 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.5mg / ml BSA
0.1mmol/l PMSF
50% Glycerol
Reaction buffer M:
500 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 70°C
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Dra I 10 x 3500U
Recognition sequence:
5 '... TTT ▼ AAA ...... 3'
3 '... AAA ▲ TTT ...... 5'
Source : Deinococcus radiophilus (ATCC 27603)
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer M
Storage buffer:
50 mmol / l KCl
50 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.5mg / ml BSA
0.1mmol/l PMSF
50% Glycerol
Reaction buffer M:
500 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 70°C
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Deinococcus radiophilus (ATCC 27603)
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 3500U
- Cut Site 5 '... TTT ▼ AAA ...... 3'
3 '... AAA ▲ TTT ...... 5' - Heat Inactivation 30 min at 70°C < 5% activity
Storage Details
- Pack Description 3500Ux10
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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