This product is for Professional Use only and not for sale to the general public for home use.
Please log in with a business account to purchase.
Restriction Endonuclease BstE II DNA Cleavage 5 x 1300U Nippon Gene Wako
Product code: 318-00095
Pack Size: 1300Ux5
CE Verified: Research Use Only
Contact us
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
Restriction Endonuclease BstE II (BstP I) produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
BstE II (BstP I) 5 x 1300U
Recognition sequence:
5 '... G ▼ GTNAC C ...... 3'
3 '... C CANTG ▲ G ...... 5'
Source : Bacillus stearothermophilus ET
Activity: 5 - 20 units / μl
Reaction Conditions: 60°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
BstE II (BstP I) 5 x 1300U
Recognition sequence:
5 '... G ▼ GTNAC C ...... 3'
3 '... C CANTG ▲ G ...... 5'
Source : Bacillus stearothermophilus ET
Activity: 5 - 20 units / μl
Reaction Conditions: 60°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 60°C
- Source Species Bacillus stearothermophilus ET
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 60°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 1300U
- Cut Site 5 '... G ▼ GTNAC C ...... 3'
3 '... C CANTG ▲ G ...... 5' - Heat Inactivation No
Storage Details
- Pack Description 1300Ux5
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
You may also be interested in
Prev
Next