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Restriction Endonuclease Bgl II DNA Cleavage 5 x 2500U Nippon Gene Wako
Product code: 315-00083
Pack Size: 2500Ux5
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Bgl II produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Bgl II 5 x 2500U
Recognition sequence:
5 '... A ▼ GATC T ...... 3'
3 '... T CTAG ▲ A ...... 5'
Source : Bacillus globigii RUB562 (Bgl I free strain)
Activity: 5-20 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
200 mmol / l KCl
10 mol / l Tris-HCl (pH 8.0)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Does not cut 5'...AGATmCT...3'.
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Bgl II 5 x 2500U
Recognition sequence:
5 '... A ▼ GATC T ...... 3'
3 '... T CTAG ▲ A ...... 5'
Source : Bacillus globigii RUB562 (Bgl I free strain)
Activity: 5-20 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
200 mmol / l KCl
10 mol / l Tris-HCl (pH 8.0)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Does not cut 5'...AGATmCT...3'.
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Bacillus globigii RUB562 ( Bgl I free strain)
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 2500U
- Cut Site 5 '... A ▼ GATC T ...... 3'
3 '... T CTAG ▲ A ...... 5' - Heat Inactivation No
Storage Details
- Pack Description 2500Ux5
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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