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Restriction Endonuclease Afl II DNA Cleavage 200U Nippon Gene Wako
Product code: 312-02531
Pack Size: 200U
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Aft II produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Aft II 200U
Recognition sequence:
5 '...... C ▼ TTAA G ...... 3'
3 '...... G AATT ▲ C ...... 5'
Source : Anabaena flosaquae
Activity: 1-10 units / μl
Reaction Conditions: 37°C in 1x Buffer A
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.4)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Buffer A 10X:
500 mmol / l Potassium acetate
200 mmol / l Tris-acetate (pH 7.9 at 25 ° C)
100 mmol / l Magnesium acetate
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 65°C
Heat inactivation must also take into consideration the DNA denaturation tempearature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Aft II 200U
Recognition sequence:
5 '...... C ▼ TTAA G ...... 3'
3 '...... G AATT ▲ C ...... 5'
Source : Anabaena flosaquae
Activity: 1-10 units / μl
Reaction Conditions: 37°C in 1x Buffer A
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.4)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Buffer A 10X:
500 mmol / l Potassium acetate
200 mmol / l Tris-acetate (pH 7.9 at 25 ° C)
100 mmol / l Magnesium acetate
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 65°C
Heat inactivation must also take into consideration the DNA denaturation tempearature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Anabaena flosaquae
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 1 - 10 units / μl
- Concentration 200U
- Cut Site 5 '...... C ▼ TTAA G ...... 3'
3 '...... G AATT ▲ C ...... 5' - Heat Inactivation 30 min at 65°C < 5% activity
Storage Details
- Pack Description 200U
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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