Fluorescein diacetate FDA Cell Analysis Live Cell Stain 1mg Cellstain® Dojindo Laboratories Wako

Cellstain® FDA produced by Dojindo Laboratories and distributed in association with FUJIFILM Wako Chemicals Europe GmbH

The Cellstain® range, produced by Dojindo Laboratories is intended for research use to facilitate cell analysis. Cellstain® Fluorescein diacetate (FDA) is cell-membrane permeable and accumulates inside of viable cells as fluorescein. Since fluorescein is less hydrophilic than BCECF or Calcein, the leakage of fluorescein from cells is rather high. FDA is also utilized for flow cytometry. The excitation and emission wavelengths of fluorescein are 488 nm and 530 nm, respectively.

Staining Procedure

1. Prepare 0.5 mg/ml FDA stock solution with DMSO. Dilute 10 µl of the stock solution with 5 ml PBS(-).
2. Prepare a cell suspension and wash cells with PBS(-). Prepare 1x10⁵-1x10⁶ cells/ml cell suspension
3. Add 15 µl FDA solution to 30 µl cell suspension, and incubate at 37°C for 15-30 min.
4. Put 10 µl stained cell suspension on a glass slide and cover with a cover glass.
5. Observe the cells under a fluorescence microscope with 488 nm excitation and 530 nm emission filters.

Cellstain® FDA 1mg
Dojindo Laboratories: F209-10
Molecular Formula:C₂₄H₁₆O₇
Molecular Weight:416.38
CAS No: 596-09-8
Store -20°C, protect from light
Ships at ambient