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CFSE Carboxyfluorescein succinimidyl ester Cell Analysis Live Cell Stain 1mg Cellstain® Dojindo Laboratories Wako

Product code: 341-07401

Pack Size: 1mg
CE Verification: Research Use Only
List Price
£235.40

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Product Information

Cellstain® CFSE 1mg produced by Dojindo Laboratories and distributed in association with FUJIFILM Wako Chemicals Europe GmbH


The Cellstain® range, produced by Dojindo Laboratories is intended for research use to facilitate cell analysis. Cellstain® Carboxyfluorescein succinimidyl ester (CFSE) is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins. Hydrolysed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively.

Staining Procedure
Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 µM CFSE solution with PBS or an appropriate buffer.
1. Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.
2. Incubate the cell at 37°C for 15 to 30 min.
3. Wash cells twice with PBS or an appropriate buffer.
4. Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.
Cellstain® CFSE 1mg
Also known as 5- or 6-(N-Succinimidyloxycarbonyl)-fluorescein 3',6' diacetate
Dojindo Laboratories: C375-10
Pack Size: 1mg
Molecular Formula:C29H19NO11
MW: 557.46
CAS No: 150347-59-4
Store -20°C
Ships at ambient temperature
Reference
1. M. Bronner-Fraser, Alterations in Neural Crest Migration by a Monoclonal Antibody That Affects Cell Adhesion. J Cell Biol. 1985;101:610-617.
2. A. Nose, et al., A Novel Cadherin Cell Adhesion Molecule:Its Expression Patterns Associated WithImplantation and Organogenesis of Mouse Embryos. J Cell Biol. 1986;103:2649-2658.
3. S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.
4. C. K. Raymond, et al., Molecular Analysis of the Yeast VPS3 Gene and the Role of Its Product in Vacuolar Protein Sorting and Vacuolar Segregation during the Cell Cycle. J Cell Biol. 1990;111:877-892.
5. G. Radcliff, et al., Quantification of Effector/Target Conjugation Involving Natural Killer(NK) or Lymphokine Activated Killer(LAK) Cells by Two-color Flow Cytometry. J Immunol Methods. 1991;139:281-292.
6. S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.
7. L. S. D. Clerck, et al., Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. J Immunol Methods. 1994;172:115-124.

Specification

  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Contents Cellstain® CFSE 1mg

Storage Details

  • Pack Description 1mg
  • Shipping Condition Ambient
  • Storage Condition -20
  • Pack Length (m) 0.035
  • Pack Width (m) 0.035
  • Pack Height (m) 0.08
  • Pack Weight (kg) 0.00

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