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CFSE Carboxyfluorescein succinimidyl ester Cell Analysis Live Cell Stain 1mg Cellstain® Dojindo Laboratories Wako
Product code: 341-07401
- List Price
- £235.40
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Call UsProduct Information
The Cellstain® range, produced by Dojindo Laboratories is intended for research use to facilitate cell analysis. Cellstain® Carboxyfluorescein succinimidyl ester (CFSE) is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins. Hydrolysed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively.
Staining Procedure
Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 µM CFSE solution with PBS or an appropriate buffer.
1. Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.
2. Incubate the cell at 37°C for 15 to 30 min.
3. Wash cells twice with PBS or an appropriate buffer.
4. Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.
Cellstain® CFSE 1mg
Also known as 5- or 6-(N-Succinimidyloxycarbonyl)-fluorescein 3',6' diacetate
Dojindo Laboratories: C375-10
Pack Size: 1mg
Molecular Formula:C29H19NO11
MW: 557.46
CAS No: 150347-59-4
Store -20°C
Ships at ambient temperature
Reference
1. M. Bronner-Fraser, Alterations in Neural Crest Migration by a Monoclonal Antibody That Affects Cell Adhesion. J Cell Biol. 1985;101:610-617.
2. A. Nose, et al., A Novel Cadherin Cell Adhesion Molecule:Its Expression Patterns Associated WithImplantation and Organogenesis of Mouse Embryos. J Cell Biol. 1986;103:2649-2658.
3. S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.
4. C. K. Raymond, et al., Molecular Analysis of the Yeast VPS3 Gene and the Role of Its Product in Vacuolar Protein Sorting and Vacuolar Segregation during the Cell Cycle. J Cell Biol. 1990;111:877-892.
5. G. Radcliff, et al., Quantification of Effector/Target Conjugation Involving Natural Killer(NK) or Lymphokine Activated Killer(LAK) Cells by Two-color Flow Cytometry. J Immunol Methods. 1991;139:281-292.
6. S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.
7. L. S. D. Clerck, et al., Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. J Immunol Methods. 1994;172:115-124.
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Contents Cellstain® CFSE 1mg
Storage Details
- Pack Description 1mg
- Shipping Condition Ambient
- Storage Condition -20
- Pack Length (m) 0.035
- Pack Width (m) 0.035
- Pack Height (m) 0.08
- Pack Weight (kg) 0.00