Gellan Gum powder plant tissue culture media semi solid 250g Wako

Gellan gum 250g produced by FUJIFILM Wako Pure Chemical Corporation.


Gelling agents such as gellan gum are routinely used to prepare solid or semi-solid plant tissue culture media. Gellan gum consists of polysaccharides produced by Pseudomonas elodea, deacetylated and purified, and its active components are glucose, rhamnose and uronic acid.

1. It offers clear solid medium after gelling due to a higher clarity than agar allowing easy observation inside the medium.

2. Soluble salts are required for gelling. Therefore, the hardness of the resulting gel can be modified by the choice of salt and volume added to the preparation in addition to varying the amount of gellan gum.

3. A smaller amount of gellan gum offers gel strength equal to agar.

4. It can accelerate growth rate of plant bodies.

5. It is heat-resistant, not solating at high temperatures, allowing autoclave sterilization several times.

6. Similarly to agar, it melts by heating (90 degrees C minimum) and gelates by cooling (30 to 50 degrees C maximum).

This product is a dried powder of polysaccharides produced extracellularly by Pseudomonas elodea, deacetylated and purified, and it is used as a gelling agent replacing agar in the culture media for plant tissues and microorganisms. It becomes a transparent gel at a lower concentration of 0.2 to 0.4 % as compared to agar and is said to offer good callus growth.

250g gellan gum powder for plant tissue culture

pH (10g/l aqueous slurry, 25C): 4.0-7.0

Store at room temperature

CAS: 71010-52-1