Phosphorylated Tau T181 ELISA Kit for human CSF produced by FUJIFILM Wako Chemical Corporation

Phosphorylated Tau T181 ELISA Kit produced by FUJIFILM Wako Chemical Corporation

Tau is a microtubule-associated protein. It is mainly expressed in neurons of the central nervous system and regulates the stability of microtubules. In the brain of patients with Alzheimer’s disease, phosphorylated Tau aggregates and forms neurofibrillary tangles (NFTs). There is evidence suggesting that the extent of NFTs correlates with the severity of dementia. Therefore, Tau has been studied extensively to better understand the causes of Alzheimer’s disease and to develop novel therapeutics.
As the concentration of Tau and phosphorylated Tau in cerebrospinal fluid (CSF) of Alzheimer’s disease patients is higher than that of non-dementia patients, it is important to measure the concentration of Tau and phosphorylated Tau in CSF. This highly sensitivity ELISA kit measures Tau including phosphorylated threonine 181 (pT181) in CSF.


Kit contents:
Tau Antibody coated plate, 1 plate 96 wells（8 × 12）
Phosphorylated Tau Standard, 1 tube (standard stock- 10ng/ml), Use after reconstitution
Buffer 60ml/1 bottle
Biotin-conjugated Antibody Solution 100 µL/1 tube
Peroxidase-conjugated Streptavidin Solution 100 µL
Luminescent Reagent 1 6 ml/1 tube
Luminescent Reagent 2 6ml/1 tube
Wash Solution concentrate (20x) 50ml/1 bottle
Standard buffer (blue color) 12ml/1 tube
Sample Buffer (red color) 50ml/ 1 bottle
Plate seal 3 sheets
Instruction manual 1 copy

Sample type: Human cerebrospinal fluid (CSF)
Sample volume: 20µl
Assay time: approx. 20 hours Detection method: Luminescence method Dynamic range: 4.40-500 pg/mL


Store at 2-10C.
For Research Use Only.