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Immunofluorescent probe neurofibrillary plaque visualisation BF-170 Wako
Product code: 026-16371
Pack Size: 1mg
CE Verified: Research Use Only
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Product Information
BF-170 Probe produced by FUJIFILM Wako Chemical Corporation
Neurofibrillary tangles form in the brains of Alzheimer's sufferers. The Wako BF-170 probe exhibits a greater affinity for Tau fibrils, neuropil threads and paired helical filament than to amyloid beta fibrils. BF-170 is soluble in methanol or ethanol an has an excitation wavelength of 450-490nm and emission wavelength >520nm.
Staining Method:
Deparaffinise the section on a glass slide
Immerse the section in 10% formalin neutral buffer for 60 minutes
Wash the section with PBS for 5 minutes
Blanch the section with 0.25% potassium permanganate solution for 20 minutes
Wash the section twice with PBS for 2 minutes
Immerse the section in 0.1% potassium metabisulphite and 0.15% oxalic acid for a few seconds
Wash the section three times with PBS for 2 minutes
Immerse the quenched tissue section with 100µmol/L BF-170 solution containing 50% ethanol for 10 minutes
Dip the section in water five times, immersing for a few seconds.
Examine the stained section using a fluorescence microscope.
BF-170 Probe 1mg
Molecular formula: C15H12N2
Molecular weight: 220.27
Store -20°C
Neurofibrillary tangles form in the brains of Alzheimer's sufferers. The Wako BF-170 probe exhibits a greater affinity for Tau fibrils, neuropil threads and paired helical filament than to amyloid beta fibrils. BF-170 is soluble in methanol or ethanol an has an excitation wavelength of 450-490nm and emission wavelength >520nm.
Staining Method:
Deparaffinise the section on a glass slide
Immerse the section in 10% formalin neutral buffer for 60 minutes
Wash the section with PBS for 5 minutes
Blanch the section with 0.25% potassium permanganate solution for 20 minutes
Wash the section twice with PBS for 2 minutes
Immerse the section in 0.1% potassium metabisulphite and 0.15% oxalic acid for a few seconds
Wash the section three times with PBS for 2 minutes
Immerse the quenched tissue section with 100µmol/L BF-170 solution containing 50% ethanol for 10 minutes
Dip the section in water five times, immersing for a few seconds.
Examine the stained section using a fluorescence microscope.
BF-170 Probe 1mg
Molecular formula: C15H12N2
Molecular weight: 220.27
Store -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Source Species NULL
- CAS Number NULL
- Application Detection of neurofibrillary tangles in tissue sections for Alzheimer's research
- Form 1mg
- Concentration NULL
- Purity NULL
- Marketed Grade NULL
- Molecular Weight 220.27
- Shelf Life NULL
- Species Reactivity NULL
- Preparation Form NULL
Storage Details
- Pack Description 1mg
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.3
- Pack Width (m) 0.3
- Pack Height (m) 0.3
- Pack Weight (kg) 0.00