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BF-188 Amyloid β & neurofibrillary tangle fluorescent probe Wako
Product code: 025-18801
Pack Size: 1mg
CE Verified: Research Use Only
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Product Information
BF-188 Amyloid β & neurofibrillary tangle fluorescent probe produced by FUJIFILM Wako Chemical Corporation
BF-188 is a flourescence probe that stains both amyloid β plaques and neurofibrillary tangles. Both plaques and tangles can be visualised under a blue-violet filter (400-440 nm) with BF-188 staining. Amyloid β plaques and neurofibrillary tangles can be detected selectively under violet (380-420nm) and green (510-560nm) filters respectively.
Soluble in methanol, BF-188 has an excitation wavelength of 400-440nm and emission wavelength typically 520nm and 600nm.
Staining Method:
Deparaffinise the section on a glass slide
Wash the section with MQ for 10 minutes
Blanch the section with 0.25% potassium permanganate solution for 20 minutes
Wash the section twice with PBS for 2 minutes
Immerse the section in 0.1% potassium metabisulphite and 0.15% oxalic acid for a few seconds
Wash the section three times with PBS for 2 minutes
Immerse the quenched tissue section with 100µmol/L BF-188 solution containing 50% ethanol for 10 minutes
Dip the section in water five times, immersing for a few seconds.
Examine the stained section using a fluorescence microscope.
BF-188 Probe 1mg
Molecular formula: C18H17N3
Molecular weight: 275.35
Store -20°C
BF-188 is a flourescence probe that stains both amyloid β plaques and neurofibrillary tangles. Both plaques and tangles can be visualised under a blue-violet filter (400-440 nm) with BF-188 staining. Amyloid β plaques and neurofibrillary tangles can be detected selectively under violet (380-420nm) and green (510-560nm) filters respectively.
Soluble in methanol, BF-188 has an excitation wavelength of 400-440nm and emission wavelength typically 520nm and 600nm.
Staining Method:
Deparaffinise the section on a glass slide
Wash the section with MQ for 10 minutes
Blanch the section with 0.25% potassium permanganate solution for 20 minutes
Wash the section twice with PBS for 2 minutes
Immerse the section in 0.1% potassium metabisulphite and 0.15% oxalic acid for a few seconds
Wash the section three times with PBS for 2 minutes
Immerse the quenched tissue section with 100µmol/L BF-188 solution containing 50% ethanol for 10 minutes
Dip the section in water five times, immersing for a few seconds.
Examine the stained section using a fluorescence microscope.
BF-188 Probe 1mg
Molecular formula: C18H17N3
Molecular weight: 275.35
Store -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Source Species NULL
- CAS Number NULL
- Application Immunofluorescence
- Molecular Formula C18H17N3
- Form 1mg
- Concentration NULL
- Purity NULL
- Marketed Grade NULL
- Molecular Weight 275.35
- Shelf Life NULL
- Species Reactivity NULL
- Preparation Form NULL
Storage Details
- Pack Description 1mg
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.015
- Pack Width (m) 0.015
- Pack Height (m) 0.022
- Pack Weight (kg) 0.00