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IgE

Variability Free IgE

The unique DIA HE1 IgE preparation from BioPorto, is the only commercially available human monoclonal IgE antibody.

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Very Low Batch-to-Batch Variation

The BioPorto Protein L purified human IgE antibody with kappa light chains is produced in vitro from a monoclonal hybridoma. This unique source guarantees freedom from contamination by other immunoglobulin isotypes. The purity of the human IgE is > 90%, tested by SDS-PAGE.

The monoclonal source of the IgE antibody ensures very low batch-to-batch variation which renders it suitable as a standard in IgE quantifying assays.
Other applications include immunochemistry, western blot and cellular immunology research.

The human IgE can be used in a variety of cell stimulation assays, including cell types such as:

    • human airway smooth muscle (HASM) cells 
    • mast cells (MC) 
    • human bronchial/tracheal smooth muscle (B/TSM) cells 
    • acute myelogenous leukemia (AML) blasts 
    • human neutrophils

Low Endotoxin

The human IgE (non-immune)(azide-free, low endotoxin) product has endotoxin levels < 25EU/mg.

The DIA HE1 IgE preparation is a Protein L purified, fully human IgE antibody with kappa light chains. The IgE is produced in vitro from a monoclonal hybridoma.

This unique source guarantees freedom from contamination by other immunoglobulin isotypes.

IgE Purity

Lane 1: molecular weight markers. 

Lane 2
: DIA HE1 purified human IgE, 185 kDa. 

Lane 3
: IgE, purified from serum from a myeloma patient 

  • Human IgE (Non-immune, low endotoxin)

    • PDF size: 10.71KB
    • Uploaded on: 18/09/2015

    Product Specification and Scientific References

    Open File

Proven Quality

Testimony to the quality of the BioPorto IgE product is its cited use  in a number of high-quality studies published in the scientific literature e.g.

1. Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset).
Nunomura S et al. Int Immunol. 2012 Sep;24(9):593-603. 

2. Proinflammatory and Th2 cytokines regulate the high affinity IgE receptor (FcepsilonRI) and IgE-dependant activation of human airway smooth muscle cells. 
Redhu NS et al.  PLoS One. 2009 Jul 7;4(7):e6153

3. IgE induces proliferation in human airway smooth muscle cells: role of MAPK and STAT3 pathways. Redhu NS et al.
Allergy Asthma Clin Immunol. 2013 Oct 17;9(1):41.

Additional scientific references are available in the Product Specification sheet that can be downloaded from the adjacent literature tab.

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