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Lysyl endopeptidase® LysC digestive enzyme Mass Spectrometry MS grade lysobacter in gel digest Wako

Product code: 125-05061

Pack Size: 20µgx5
CE Verified: Research Use Only

Product Information

lysyl endopeptidase LysC digestive enzyme Mass Spectrometry MS grade manufactured by FUJIFILM Wako Pure Chemical Corporation.

Proteins separated by electrophoresis may be excised from polyacrylamide gels and subject to in-gel digestion with proteolytic enzymes in preparation for further downstream analysis such as mass spectrometry for peptide identification and analysis of post-translation modifications.

The source of this lysyl endopeptidase was originally indicated as “Achromobacter lyticus” based on the physiological and morphological properties of the bacteria. However, the 16sRDNA sequence was found to be highly homologous to that of Lysobacter.

This enzyme is suitable grade for mass spectrometry work.

Lysyl Endopeptidase specifically cleaves the peptides on the carboxyterminal side of lysine residues. (C terminal Lys). Use alone or in combination with Trypsin (C terminal Arg and Lys cleavage) for in-gel digest of protein samples prior to analysis by MALDI-TOF or LS-MS.

Molecular weight 27,500
Activity 0.03 – 0.07 AU/vial

Store at -20C.


  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Specific Activity Specifically cleaves the peptides on the carboxy-terminal side of lysine residues.
  • Purity NULL
  • Concentration 0.03-0.07AU/vial
  • EC Number
  • Optimal Temperature 30C
  • Optimal pH 9.0-9.5
  • Source Species soil bacterium
  • Application in gel digest, Mass Spectrometry sample preparation
  • CAS Number NULL
  • Unit Definition One amidase unit (AU) is the amount of enzyme which will produce 1µmol of p-nitroaniline per minute 30C, pH 9.5.

Storage Details

  • Pack Description 20µgx5
  • Shipping Condition Dry Ice
  • Storage Condition -20
  • Pack Length (m) 0.02
  • Pack Width (m) 0.015
  • Pack Height (m) 0.0018
  • Pack Weight (kg) 0.50

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