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Faecal Calprotectin ELISA BÜHLMANN fCAL® for differentiating IBS vs IBD and disease monitoring. EK-CAL2 2x96 Wells
Pack size: 2 x 96Well
Product code: EK-CAL2

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Faecal Calprotectin ELISA BÜHLMANN fCAL® for differentiating IBS vs IBD and disease monitoring. EK-CAL2 2x96 Wells

Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.

Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.

The BÜHLMANN fCAL® ELISA is ideal for use in high through put laboratory environments for screening and monitoring IBD patients:

Code: EK-CAL2

Sample Type: Faeces

Test Time: 75 minutes

Assay Range: 30 - 1800µg/g or 10 - 600µg/g (Different test protocol to achieve the higher or lower range)

Number of wells: 2 x 96 Each plate is individually sealed (up to 80 samples in duplicate)

Assay protocols: DS2 and Triturus

All reagents are ready to use (except the wash buffer)

Calibrators and controls are included

Analytical Sensitivity: < 10µg/g Calprotectin in faecal sample

Established cut-off of 50µg/g
Brand
BÜHLMANN
Sterile
No
CE Certified
CE IVD
Warranty Period
Standard Terms Apply
Shelf Life
minimum 6 months
Sensitivity
10µg/g
Application
quantification of faecal calprotectin for differentiation between IBD and IBS; patient monitoring mucosal healing
Specificity
cross reactivity < 0.1%
Contents
6x125ml extraction buffer; 2x12x8 wells pre-coated with anti-calprotectin mAb;6xplate seals;2x100ml wash buffer concentrate (10x)(dilute before use);3 x125ml incubation buffer; Calibrators A-E 5x1ml; Controls low and high 2x1ml; 2x12ml Anti-Calprotectin Ab conjugated to HRP;2x12ml TMB substrate; 2x12ml Stop Solution
Time Required
75min
UNSPSC Description
Chemistry test kits or supplies
UNSPSC Code
41116104
NHS eClass Description
Chemical & Biochemical Diagnostic Products
NHS eClass Code
HHB
Pack Description
2 x 96Well
Shipping Condition
Ambient
Storage Condition
Fridge
Pack Length (m)
0.22
Pack Width (m)
0.16
Pack Height (m)
0.11
Pack Weight (Kg)
0.2000

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  • 10 Years of Calprotectin Testing in the UK

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  • When is the Best Time for Sampling Faeces for Calprotectin Analysis?

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    Turvill J et al., Department of Gastroenterology, York Teaching Hospital NHS Foundation Trust, Online First 2016

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