STAT-NAT® Malaria Typing Kit manufactured by Sentinel Diagnostics.
Parasites of the genus Plasmodium are the causative agent of Malaria. Infected mosquitoes can pass on the Plasmodium parasite via their saliva when feeding on humans or animals and act as a disease vector spreading infection from one host to another. On entering the blood stream the Plasmodium sporozoites migrate to the liver where they mature and reproduce. Species of Plasmodium that cause malaria in humans include P. falciparum, P. vivax, P.ovale and P.malariae; of these it is P.faciparum that causes the majority of fatal malaria infections. Rapid identification of the infecting species in order to provide the correct treatment has the greatest impact on prognosis in cases of severe malaria (produced by P. falciparum or mixed infection including P. falciparum).
This kit is the ideal companion to the STAT-NAT Malaria screening kit (1N051). The screening kit provides a positive diagnosis of the presence of Plasmodia in a sample and confirms the presence of P.falciparum. However if screening indicates that a Plasmodium species other than P.falciparum is present the typing kit can be used to identify it. The typing kit is a qualitative test that detects the presence of 3 of the 4 Plasmodia that infect humans (P. malariae, P. ovale and P. vivax) in blood samples but does not detect P. falciparum. This allows the Typing kit to be used to verify and extend the information provided by the screening kit. STAT-NAT Malaria Typing kit
The STAT-NAT stabilised amplification technology enables production of a thermally stable, lyophilised PCR assay mix containing Hot Start Polymerase, magnesium chloride (MgCl2), primers and dNTPs. The optimised PCR assay mix is pre-aliquoted into strips of 8x0.2ml PCR tubes and can be stored at 15-30C so will not take up valuable cold storage space. To run the assay just add DNA and sterile water to a final volume of 25µl and proceed with the PCR programme