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Real-Time PCR for Antimicrobial Resistance Detection

Identification of resistance mechanisms is key in controlling the spread of infection and can help determine the best therapeutic options for patients.

Nucleic acid tests supplement the conventional culture susceptibility testing in the identification of theses mechanisms.

Real Time PCR involves extraction of DNA from bacterial cells, amplification of the genetic material and subsequent detection using real-time PCR techniques.

Antibiotic resistance is considered a global threat to public health that is likely to spread within populations. It is responsible for increasing rates of mortality and represents a significant challenge to the NHS.

β-lactamases, enzymes that inactivate the β-lactam family of antibiotics and render them ineffective for treatment of clinically-important Gram-negative bacterial infections, confer resistance to penicillins, cephamycins, and in some cases, carbapenems.

β-lactam-resistant Gram-negative organisms producing multiple or plasmid-mediated β-lactamases are difficult to identify phenotypically and necessitate more specific detection methods to identify clinically important β-lactamases. Genetic identification of these resistance mechanisms is critical for active surveillance and infection control. Because these antibiotics are often selected for the management and prevention of infectious disease, the presence and characteristics of specific β-lactamases play a critical role in selecting the appropriate antibiotic therapy.

The Streck ARM-D® kits are multiplex real-time PCR kits for the detection of clinically-relevant β-lactamase genes.

Streck ARM-D® Kit, β-Lactamase detects 9 different Carbapenemases, ESBLs and plasmid-mediated ampC gene families.

The Streck ARM-D® Kit, ampC kit targets 6 plasmid-mediated ampC resistance genes and can differentiate plasmid-mediated ampC β-lactamase resistance from chromosomal resistance.

The Streck ARM-D® kits complement phenotypic methods for the detection of multidrug resistant organisms. 

The kits can be used with most 4-channel real-time PCR instruments, and have been validated for use with the following systems:  Applied Biosystems 7500 Fast, Applied Biosystems QuantStudio® 7Flex, Bio-Rad® CFX96 Touch™, QIAGEN Rotor-Gene Q and Streck Zulu RT™.

  • Comprehensive list of clinically-relevant β-lactamase target genes (Carbapenemases, ESBLs and plasmid-mediated ampC gene families) that complements phenotypic methods for the detection of multidrug resistant organisms,
  • Detection and discrimination between plasmid-mediated and chromosomally expressed AmpC β-lactamases,
  • Improved surveillance and epidemiologic tracking leading to better infection control and increased effectiveness of antibiotic stewardship programs,
  • Improved management and prevention of infectious diseases – selection of appropriate antibiotic treatment.  

  • ARM-D Flyer

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    Streck ARM-D Kits Flyer

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  • ARM-D Frequently Asked Questions

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    FAQs

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  • ARM-D Kit ampC (RUO) IFU

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    Instructions for Use

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  • ARM-D Kit β-Lactamase (RUO) IFU

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    Instructions for Use

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  • ARM-D Kits Poster

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    • Uploaded on: 16/02/2017

    Detection of ESBLs, MBLs, KPCs, and Plasmid-Mediated ampC β-Lactamase Genes by Multiplex Real-Time PCR

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  • ARM-D Kits Technical Note

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    A multiplex real-time PCR assay for identification of multi-drug resistant Gram-negative pathogens

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  • ARM-D Kits Technical Note

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    Plasmid-mediated β-Lactamases: Nomadic Microbial Assassins Lurking in Multidrug Resistant Gram-negative Strains

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  • New Antimicrobial Resistance Molecular Tests

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    • Uploaded on: 22/03/2017

    Streck ARM-D Kits Article

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