The pancreatic elastase ELISA from BIOSERV Diagnostics is for the in vitro quantitative determination of human pancreatic elastase in faeces, to aid in the diagnosis of exocrine pancreatic function.
For many labs, the faecal elastase assay is the preferred test used to screen for exocrine pancreatic insufficiency; the test is easy to perform, and convenient for patients as only one sample is required and the test is non-invasive. The faecal elastase assay has replaced the gold standard faecal fat test in most laboratories.
Pancreatic elastase is a proteolytic enzyme, it’s produced exclusively in the pancreas and is highly stable throughout the intestinal passage.
Due to its high stability, the concentration of this enzyme can be measured in faeces to reflect the exocrine secretory capacity of the pancreas.
The pancreatic elastase ELISA from BIOSERV Diagnostics is an easy to use, non-invasive method to quantitatively determine the concentration of pancreatic elastase in faeces.
This assay can be used to help diagnose exocrine pancreatic insufficiency, characterised by a low concentration of pancreatic elastase in faeces. Exocrine pancreatic insufficiency can be caused by numerous conditions, including: chronic pancreatitis, cystic fibrosis, diabetes mellitus, cholelithiasis, hereditary pancreatitis, chronic inflammatory bowel disease, pancreatic carcinoma, autoimmunologically caused pancreatitis, Shwachman-Diamond syndrome, autoimmune and connective tissue diseases, and Zollinger-Ellison syndrome.
The pancreatic elastase ELISA is CE IVD certified with a test range of 50-500 µg/g.
For those wishing to measure lower concentrations, we can also offer the SK15 version with an additional standard at 15 µg/g (test range 15-500 µg/g). The SK15 version of the ELISA kit is useful for those wishing to monitor patients with cystic fibrosis, to diagnose very low functioning of the exocrine pancreas and to recommend suitable therapies for these patients.
Both kits can be run manually or automated on a commercial ELISA processor, such as the Dynex DS2TM. Alpha Laboratories can offer a full protocol to run the BIOSERV pancreatic elastase ELISA on the Dynex DS2.