Creatine Kinase CK activity assay kinetic method IFCC serum plasma 3.0-1000.0U/L dual liquid reagent system CK Nac Sentinel Diagnostics

CK Nac Creatine Kinase CK activity assay manufactured by Sentinel Diagnostics

Creatine kinase (CK) is an enzyme found it the heart, brain and skeletal muscle which catalyses the reversible phosphorylation of creatine by ATP. Creatine Kinase is a dimer composed of two subunits and there are three active isoenzymes: BB (CK-1), MB (CK-2) and MM (CK-3). CK-BB is found predominantly in the brain, CK-MB mainly in the heart and CK-MM is found in the heart and other muscles. Elevated CK values are the result of muscular damage and associated pathologies and are used for diagnosis and follow-up of acute myocardial infarction and some muscular diseases.

CK-NAC Liquid contains ready-to-use reagents for determining Creatine Kinase activity in serum or plasma (heparin, EDTA) in accordance with the IFCC recommendations. Creatine Kinase catalyses the reaction between creatine phosphate and ADP which forms creatine and ATP. In the presence of glucose and hexokinase (HK) the ATP formed in the first reaction will be converted to ADP and glucose-6-phosphate. The glucose-6-phosphate then subsequently reacts with beta-NADP+ in the presence of glucose-6-phosphate dehydrogenase (G6P-DH) to form 6-phosphogluconate and beta-NADPH. Conversion of beta-NADP+ to beta-NADPH results in an increase in absorbance at 340nm and this change in absorbance is proportional to the creatine kinase (CK) activity in the sample. The reaction mixture contains N-Acetyl-L Cysteine (NAC) to allow optimal CK enzyme activation.

The CK-NAC Liquid reagents are ready-to-use and the assay has a measuring range of 3-1000 U/L. The assay should be used with 16550 Clinical Chemistry Calibrator. Store reagents at 2-8C, on board stability 30 days and calibration interval 30 days. The recommended QCs for this test are the Clinical Chemistry Controls (16150 & 16250).