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Anti-Iba1 rabbit polyclonal antibody conjugated with red fluorochrome 635 for immunocytochemistry mouse rat c-terminus specific microglia Wako
Product code: 013-26471
Pack Size: 100µl
CE Verified: Research Use Only
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Product Information
Anti-Iba1 Red Fluorochrome 635 conjugate manufactured by FUJIFILM Wako Pure Chemical Corporation.
Ionized calcium binding adaptor molecule 1 or Iba1 is specifically expressed in macrophages and microglia and upregulated during cell activation. Also known as allograft-Inflammatory Factor 1 (AIF1), Iba1 has actin-bundling activity and participates in membrane ruffling and phagocytosis in activated microglia. Iba1 expression may be of interest to those studying brain injuries and diseases.
Wako’s Anti-Iba1 polyclonal primary antibody was raised in rabbit using a synthetic peptide corresponding to the C terminus of Iba1 as immunogen. Anti-Iba1 is specific to microglia and macrophages but will not cross react with neurons or astrocytes. This antibody has been conjugated with a red fluorochrome 635 like Cy5™. The use of a the pre conjugated Red fluorochrome (635) eliminates the need for a secondary antibody, therefore speeding up the staining process. Additionally, this also reduces background fluorescence.
Suggested working concentration for Immunohistochemistry is 1:200 – 2000
Reacts with mouse and rat (other species have not been tested)
Rabbit IgG
Pack size 100µl.
Store at 2-10°C.
For Research Use Only.
Ionized calcium binding adaptor molecule 1 or Iba1 is specifically expressed in macrophages and microglia and upregulated during cell activation. Also known as allograft-Inflammatory Factor 1 (AIF1), Iba1 has actin-bundling activity and participates in membrane ruffling and phagocytosis in activated microglia. Iba1 expression may be of interest to those studying brain injuries and diseases.
Wako’s Anti-Iba1 polyclonal primary antibody was raised in rabbit using a synthetic peptide corresponding to the C terminus of Iba1 as immunogen. Anti-Iba1 is specific to microglia and macrophages but will not cross react with neurons or astrocytes. This antibody has been conjugated with a red fluorochrome 635 like Cy5™. The use of a the pre conjugated Red fluorochrome (635) eliminates the need for a secondary antibody, therefore speeding up the staining process. Additionally, this also reduces background fluorescence.
Suggested working concentration for Immunohistochemistry is 1:200 – 2000
Reacts with mouse and rat (other species have not been tested)
Rabbit IgG
Pack size 100µl.
Store at 2-10°C.
For Research Use Only.
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Host Rabbit
- Clonality Polyclonal
- Application Immunohistochemistry
- Form 100#0181;l
- Specificity microglia and macrophage
- Species Reactivity mouse rat
- Preparation Form purified by affinity chromatography from rabbit antisera and conjugated with a red fluorochrome 635
- Concentration NULL
- Clone Number NULL
- Isotype IgG
- Source Species Rabbit
- Shelf Life NULL
- Cross Reactivity Does not cross react with neurons and astrocytes
- Immunogen synthetic peptide corresponding to c-terminus of Iba-1
Storage Details
- Pack Description 100µl
- Shipping Condition Blue Ice
- Storage Condition Fridge
- Pack Length (m) 0.0015
- Pack Width (m) 0.0015
- Pack Height (m) 0.0022
- Pack Weight (kg) 0.00