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Phos-tag™ Agarose AG-503 3.0ml phosphate tag phosphate-affinity purification column chromatography spin-column Nard Institute Manac Inc Wako
Pack size: 3mL
Product code: 308-93563

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Phos-tag™ Agarose AG-503 3.0ml phosphate tag phosphate-affinity purification column chromatography spin-column Nard Institute Manac Inc Wako

Phos-tag™ Agarose AG-503 3ml manufactured by Nard Institute and Manac Incorporated and distributed in association with Wako Pure Chemicals.

Phos-tagTM is a novel labelling system for analysis of phosphorylation. The Phos-Tag, developed by the Department of Functional Molecular Science at Hiroshima University, recognises the phosphate monoester group. Phos-Tag agarose is perfect for separation and purification of native phosphoproteins and phosphorylated peptides from biological samples at physiological pH. The buffer contains no reducing agent and no detergent.

Phos-tag agarose can be used to produce a phosphate affinity chromatography column to bind inorganic phosphate and various phosphate dianions bound to amino acids, sugars and lipids. The captured anionic compound dissociates from the Phos-tag at pH 3-4. The eluted phosphate rich fraction can then be subjected to further downstream analysis such as electrophoresis and western blotting. The method is based on immobilized metal affinity chromatography (IMAC) using a phosphate-binding tag molecule (a dinuclear zinc (II) complex) attached on a highly cross-linked agarose (Phos-tag Agarose). Phos-tag agarose can also be used for spin-column purification methods.

The Phos-tag Agarose is supplied as a 50% (v/v) suspension of Zn2+ phosphate binding gel (AMP2- binding site = 3-5µmol/mL gel) in 20mM Tris acetate pH 7.4 with 20% (v/v) 2-propanol.

Store at 4°C.

Research Use Only.
Brand
FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
Sterile
No
CE Certified
Research Use Only
Warranty Period
Standard Terms Apply
Application Type
phosphate affinity column chromatography purification
UNSPSC Description
Agarose gel making reagents
UNSPSC Code
41105317
NHS eClass Description
Chemicals & Reagents
NHS eClass Code
HXB
Pack Description
3mL
Shipping Condition
Direct
Storage Condition
Fridge
Pack Length (m)
0.15
Pack Width (m)
0.15
Pack Height (m)
0.22
Pack Weight (Kg)
0.1000

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