Phos-tag Labelling
Phos-tagTM is a novel labelling system for analysis of phophorylation. The Phos-Tag, developed by the Department of Functional Molecular Science at Hiroshima University, recognises the phosphate monoester group. Phos-tag can therefore take the place of conventional enzyme immunoassay and radioactive isotope methods as an agent for capturing substances with a phosphate monoester group in phosphorylation analysis techniques.
Under neutral physiological pH conditions Phos-tag binds with an anionic substituent, the bond is especially strong with phosphomonoester di-anions making Phos-tag ideal for detection of phosphorylated substances and stabilising those phosphorylated compounds which were previously difficult to measure as a result of chemical instability.
The Phos-tag technology can be used in a number of innovative methods for separation, purification and detection. Phosphorylation and dephosphorylation of proteins are of key importance in signal transduction and regulate numerous functions within living organisms, therefore Phos-tag applications may be of interest to those researching regulatory pathways in numerous fields.
Phos-tag Separation:
Phos-tag acrylamide for SDS-PAGE mobility shift detection of phosphorylated proteins
Phos-Tag Detection:
Phos-tag biotin as substitute for anti-phospho antibody in Western Blot analysis
Phos-tag Purification:
Phos-tag agarose for column chromatography purification of phosphorylated proteins.
Phos-Tag Analysis:
Phos-tag Mass Analytical kit for use in MALDI-TOF/MS to improve detection of phosphorylated molecules.
| Cat No |
Description |
Pack Size |
Price |
Add to Cart |
| 302-93561 |
Phos-Tag Agarose AG-501 |
0.5ml |
£319.00
|
|
| 308-93563 |
Phos-Tag Agarose AG-503 |
3ml |
£1,312.00
|
|
| 300-93523 |
Phos-Tag Acrylamide AAL-107 |
2mg |
£383.00
|
|
| 304-93521 |
Phos-Tag Acrylamide AAL-107 |
10mg |
£839.00
|
|
| 301-93531 |
Phos-Tag Biotin BTL-104 |
10mg |
£969.00
|
|
| 308-93541 |
Phos-Tag Biotin BTL-105 |
10mg |
£969.00
|
|
| 305-93551 |
Phos-Tag Mass Analytical Kit |
Each |
£1,312.00
|
|
Associated Links
Phosphate Affinity Chromatography
Mobility Shift Assay for Phosphorylated Proteins
Western Blot analysis of Phosphorylated Proteins
MALDI-TOF MS Phosphorylated Molecules
MRC Protein Phosphorylation Unit
PhosphoSitePlus