Molecular Biology Reagents

reagents grouped Agarose

TAE/TBE Buffers

TE Buffer

SSC Buffer

SDS Solution

TRACKING DYES FOR ELECTROPHORESIS

EDTA - ULTRA PURE

Formamide - ULTRA PURE

Glycerol - ULTRA PURE

SDS - ULTRA PURE (Sodium-Dodecyl Sulphate)

Tris Buffer (Base)

Tween 20 Surfactant - ULTRA PURE

Alpha Laboratories is proud to be able to offer you a selection of quality electrophoresis reagents. We have carefully choosen these products, mainly for horizontal gel electrophoresis, although many have broader applications. When choosing these products we have concentrated on quality and convenience as highest priority. In short, in keeping with our philosophy of supplying quality to science.

The product range includes:

Agaroses to suit many applications including low-melt agaroses for easy recovery of nucleic acids and high resolution ones for resolving the smallest of nucleic acid fragments.
Buffer solutions made ready-to-use by one simple dilution plus the security of batch to batch consistency, and stringent quality testing.
Tracking dyes and loading buffer for easy monitoring of gel runs
Ultra Pure reagents of highest quality for when you want to make your own solutions.

Agarose

Application Guide

Agarose type	Applications
Agarose Universal	Nucleic acid analytical and preparative electrophoresis Blotting Protein techniques such as radial immunodiffusion
Agarose Extra	Conventional electrophoresis Pulsed field gel electrophoresis Blotting Agarose bead preparation Cell and enzyme immobilisation
Agarose Reclaim	With the highest gelling and melting temperatures and gel strength Fragment separation in analytical and preparative electrophoresis Tissue and cell culture Viral plaque assays
Agarose Reclaim Extra	With lower gelling/melting temperatures and lower gel strength than Agarose Reclaim Capillary electrophoresis Tissue and cell culture Viral plaque assays
Agarose Reclaim Ultra	Highest resolving capacity for large DNA fragments, > 1000bp including PCR products Ideal for digestion by agarase enzymes Easy to recover large DNA fragments suitable for cloning or enzymatic processing
Agarose Micro Plus	For applications that require efficient separation of small DNA fragments and PCR products

Specification Guide

	Agarose Universal	Agarose Extra	Agarose Reclaim	Agarose Reclaim Extra	Agarose Reclaim Ultra	Agarose Micro Plus
Moisture	≤7%	≤7%	≤7%	≤5%	≤7%	≤7%
Ash	≤0.4%	≤0.25%	≤0.4%	≤0.3%	≤0.4%	≤0.35%
EEO	0.05-0.13%	≤0.12	≤0.12	≤0.10	≤0.12	≤0.12
Sulphate	≤0.1%	≤0.12%	≤0.12%	≤0.12%	≤0.1%	≤0.11%
Clarity (NTU)	≤3	≤4	≤4	-	≤4	<5
Gel Strength 1% (g/cm²)	≤1200	≤1800	-	-	-	≤4200 (4%)
Gel Strength 1.5% (g/cm²)	≥2500	≥3200	≥500	≥1000 (4%)	≥500	≥2000
Gelling Temperature 1.5%	36 ± 1.5	36 ± 1.5	24 ± 28	≤35	24 – 28	≤35.5
Melting Temperature 1.5%	88 ± 1.5	88 ± 1.5	≤65.5	≤65 (4%)	≤65.5	≤93
DNase/RNase activity	None detected	None detected	None detected	None detected	None detected	None detected
DNA resolution >1000bp	Finely resolved	Finely resolved	Finely resolved	Finely resolved	Finely resolved	Finely resolved
Gel background	Very low	Very low	Very low	Very low	Very low	Very low

Ordering Information
Description	Pack size	Product Code	Price
Agarose Universal	100g 500g	EL0014 EL0015	£71.10 £265.25
Agarose Extra	100g	EL0018	£175.10
Agarose Reclaim	25g 100g	EL0019 EL0020	£76.00 £290.50
Agarose Reclaim Extra	100g	EL0028	£223.55
Agarose Reclaim Ultra	100g	EL0031	£345.05
Agarose Micro Plus	25g 100g	EL0024 EL0025	£48.95 £188.50

TAE/TBE Buffers

gels These buffers are widely used in the preparation of nucleic acid electrophoresis gels. All convenience buffers are formulated with 18 Megohm Water and 0.2 Micron Filtration.

TAE Buffer (50x) is a concentrated solution of 2M Tris-Acetate and 100mM Sodium EDTA in distilled/deionised water (pH 8.3 at 1x concentration).

TBE Buffer is available as a 5x or 10x concentrated buffer solution of Tris-Borate-EDTA in distilled/deionised water, containing 0.089M Tris base, 0.089M boric acid (pH8.3) and 2mM Na2EDTA.

Product Code	Descriptions	Pack Size	Price
EL0077	TAE Buffer 50x conc.	1L	£44.30
EL0078	TBE Buffer 5x conc.	4L	£32.50
EL0079	TBE Buffer 10x conc.	1L	£20.60
EL0080	TBE Buffer 10x conc.	4L	£49.05

TE Buffer

TE Buffer is a 100X concentrated solution of 1 M Tris-HCl, pH 8, with 100mM EDTA. Manufactured from ultra pure reagents, this buffer is the standard for DNA and RNA purification, processing and storage.

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0081	TE Buffer 100x conc.	25ml	£50.10

SSC Buffer

loading gel SSC Buffer is widely used in nucleic acid blotting and hybridization protocols. Supplied as a 20x concentrated solution of sodium chloride-sodium citrate in solution in distilled/deionised water.

SSC Buffer Concentrate (20x) is supplied in 1 litre and 4 litre bottles containing 3M sodium chloride and 0.3M sodium citrate (pH 7.0).

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0074	SSC Buffer 20x conc.	1L	£22.90

SDS Solution

Alpha Laboratories SDS solution (20%) is formulated using the highest quality SDS, that has been further purified to remove contaminants, which can interfere with spectro-photometric analysis and cause nonspecific binding. The purity of this product ensures that reproducible protein molecular weight measurements can be made with confidence, and optimizes activity recovery in enzyme purification procedures.

The convenient pre-prepared solution eliminates the discomfort and hazards of working with powdered SDS.

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0094	SDS Solution 20%(w/v)	100ml	£21.65
EL0093	SDS Solution 20%(w/v)	450ml

TRACKING DYES FOR ELECTROPHORESIS

Tracking dyes are routinely used in both protein and nucleic acid electrophoresis to monitor the progress of electrophoresis and indicate at what point the gel should be stopped. Generally they are added to the sample prior to loading on to the gel (loading buffer). Alpha Laboratories offers 3 of the most commonly used tracking dyes together with a convenient ready-made DNA loading buffer.

Bromophenol Blue

This material is the most popular tracking dye for use in alkaline and neutral buffer systems, but is not suitable for electrophoresis of native proteins in acidic buffer conditions.

Bromophenol blue is generally added to samples at a final concentration of 0.01%.

Xylene Cyanole FF

Used as a tracking dye to monitor the progress of electrophoretic separation. This tracking dye usually migrates with DNA molecules around 5 kb, making it the tracking dye of choice for longer runs.

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0037	Bromophenol Blue	10g	£32.25
EL0097	Xylene Cyanol FF	25g	£55.85

Triple Loading Buffer

With three tracking dyes (Bromophenol Blue, Xylene Cyanol, and Orange G) Triple Dye Loading Buffer (6X) is a non-denaturing loading buffer for native polyacrylamide and agarose gel applications.

In normal type agaroses in 0.5x TBE the dye mobility is broadly unaffected over the range of 0.5 to 1.4% w/v.

The dyes run as follows (linear double standard DNA);

Orange G 50 bp
Bromophenol blue 300 bp
Xylene cyanol 4 kbp

Triple dye loading buffer contains 50% (w/v) sucrose and 40mM Tris base in distilled, deionized water.

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0084	Triple Loading Dye 6x	1.2ml	£17.30

Ultra Pure Electrophoresis Reagents

Here Alpha Laboratories details a range of reagents commonly used in the preparation of electrophoresis and blotting buffers and solutions. These are all high purity reagents specifically formulated to be suitable for electrophoresis applications.

EDTA - ULTRA PURE

gels Disodium ethylene diamine tetra acetate dihydrate. Chelating agent added to electrophoresis buffers to preserve sample integrity by disabling certain metal dependant nucleases.

Specification

Molecular Weight 372.26
Purity > 99%
Fe < 0.01%
Insolubles < 0.005%
Colour < 10 APHA (0.5M solution)
Heavy Metals < 10 ppm

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0041	EDTA	100g	£55.40

Formamide - ULTRA PURE

Molecular biology grade DNA denaturing agent, redistilled for absolute purity, deionised and packed under Nitrogen.

Used as a gel additive and as a primary component of denaturing DNA electrophoresis sample buffers. Ready-to-use.

Specification

Molecular Weight 45.04
Purity > 99%
Conductivity < 25 µmho
Heavy Metals < 5 ppm
Colour < 10 APHA µmho

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0024	Formamide	200ml	£48.95

Glycerol - ULTRA PURE

gels 4 Used in loading buffers, to modify the specific gravity of certain electrophoresis solutions, and to alter the mechanical properties of gels.

Specification

Molecular Weight 92.09
Assay > 99.7%
pH neutral
Colour < 10 APHA
A280 < 0.05

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0046	Glycerol	450ml	£54.70

SDS - ULTRA PURE (Sodium-Dodecyl Sulphate)

Used as a powerful protein denaturant and solubilising agent, which induces conformational changes in proteins at low concentrations. Enabling proteins to be separated on the basis of their molecular weight.

The purity of these products ensures that reproducible protein molecular weight measurements can be made with confidence.

Purified to remove coloured contaminants that may interfere with spectrophotometric analysis. Zero free sulphates eliminate non-specific binding.

Specification

Molecular Weight 288.38
Purity > 99%
OD280 (3% Solution) < 0.1
C12Sulphate > 99%
Lead (Pb) < 0.0002%
Phosphate (PO4) < 0.0001%

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0071	Sodium Dodecyl Sulphate	100g	£23.55
EL0070	Sodium Dodecyl Sulphate	1KG	£142.70

Tris Buffer (Base)

gels 3 Tris(hydroxymethyl)aminomethane. Buffer salt used in both protein and DNA electrophoresis. Purified to remove ammonia and amine contaminants. Results in greater resolution during isoelectric focusing.

Specification

Molecular Weight 121.14
Purity > 99.9%
Pb < 1 ppm
OD280(1M solution) < 0.15
Mg < 1 ppm
Fe < 1 ppm
As < 5 ppm
Cu < 1 ppm

Catalogue Number Description Pack Size
EL0085 Tris Buffer (Base) 250g
EL0086 Tris Buffer (Base) 1kg

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0085	Tris Buffer (Base)	250g	£26.00
EL0086	Tris Buffer (Base)	1KG	£55.15

Tween 20 Surfactant - ULTRA PURE

A Non-ionic surfactant, used for Western blotting and other molecular biology and biochemistry applications. Tween 20. Specially purified to remove epoxidized contaminants.

Product Code	Descriptions	Pack Size	Price	Add to Cart
EL0091	Tween 20 Surfactant	1L	£49.05

Tween-20 is a Registered Trademark of ICI America