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Agarose
TAE/TBE Buffers
TE Buffer
SSC Buffer
SDS Solution
TRACKING DYES FOR ELECTROPHORESIS
EDTA - ULTRA PURE
Formamide - ULTRA PURE
Glycerol - ULTRA PURE
SDS - ULTRA PURE (Sodium-Dodecyl Sulphate)
Tris Buffer (Base)
Tween 20 Surfactant - ULTRA PURE
Alpha Laboratories is proud to be able to offer you a selection of quality electrophoresis reagents. We have carefully choosen these products, mainly for horizontal gel electrophoresis, although many have broader applications. When choosing these products we have concentrated on quality and convenience as highest priority. In short, in keeping with our philosophy of supplying quality to science.
The product range includes:
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These buffers are widely used in the preparation of nucleic acid electrophoresis gels. All convenience buffers are formulated with 18 Megohm Water and 0.2 Micron Filtration.
TAE Buffer (50x) is a concentrated solution of 2M Tris-Acetate and 100mM Sodium EDTA in distilled/deionised water (pH 8.3 at 1x concentration).
TBE Buffer is available as a 5x or 10x concentrated buffer solution of Tris-Borate-EDTA in distilled/deionised water, containing 0.089M Tris base, 0.089M boric acid (pH8.3) and 2mM Na2EDTA.
TE Buffer is a 100X concentrated solution of 1 M Tris-HCl, pH 8, with 100mM EDTA. Manufactured from ultra pure reagents, this buffer is the standard for DNA and RNA purification, processing and storage.
SSC Buffer is widely used in nucleic acid blotting and hybridization protocols. Supplied as a 20x concentrated solution of sodium chloride-sodium citrate in solution in distilled/deionised water.
SSC Buffer Concentrate (20x) is supplied in 1 litre and 4 litre bottles containing 3M sodium chloride and 0.3M sodium citrate (pH 7.0).
Alpha Laboratories SDS solution (20%) is formulated using the highest quality SDS, that has been further purified to remove contaminants, which can interfere with spectro-photometric analysis and cause nonspecific binding. The purity of this product ensures that reproducible protein molecular weight measurements can be made with confidence, and optimizes activity recovery in enzyme purification procedures.
The convenient pre-prepared solution eliminates the discomfort and hazards of working with powdered SDS.
Tracking dyes are routinely used in both protein and nucleic acid electrophoresis to monitor the progress of electrophoresis and indicate at what point the gel should be stopped. Generally they are added to the sample prior to loading on to the gel (loading buffer). Alpha Laboratories offers 3 of the most commonly used tracking dyes together with a convenient ready-made DNA loading buffer.
Used mainly as a tracking dye for DNA electrophoresis in agarose and for native protein electrophoresis with acidic buffers.
This material is the most popular tracking dye for use in alkaline and neutral buffer systems, but is not suitable for electrophoresis of native proteins in acidic buffer conditions.
Bromophenol blue is generally added to samples at a final concentration of 0.01%.
Used as a tracking dye to monitor the progress of electrophoretic separation. This tracking dye usually migrates with DNA molecules around 5 kb, making it the tracking dye of choice for longer runs.
With three tracking dyes (Bromophenol Blue, Xylene Cyanol, and Orange G) Triple Dye Loading Buffer (6X) is a non-denaturing loading buffer for native polyacrylamide and agarose gel applications.
In normal type agaroses in 0.5x TBE the dye mobility is broadly unaffected over the range of 0.5 to 1.4% w/v.
The dyes run as follows (linear double standard DNA);
Triple dye loading buffer contains 50% (w/v) sucrose and 40mM Tris base in distilled, deionized water.
Here Alpha Laboratories details a range of reagents commonly used in the preparation of electrophoresis and blotting buffers and solutions. These are all high purity reagents specifically formulated to be suitable for electrophoresis applications.
Disodium ethylene diamine tetra acetate dihydrate. Chelating agent added to electrophoresis buffers to preserve sample integrity by disabling certain metal dependant nucleases.
Specification
Molecular biology grade DNA denaturing agent, redistilled for absolute purity, deionised and packed under Nitrogen.
Used as a gel additive and as a primary component of denaturing DNA electrophoresis sample buffers. Ready-to-use.
Used in loading buffers, to modify the specific gravity of certain electrophoresis solutions, and to alter the mechanical properties of gels.
Used as a powerful protein denaturant and solubilising agent, which induces conformational changes in proteins at low concentrations. Enabling proteins to be separated on the basis of their molecular weight.
The purity of these products ensures that reproducible protein molecular weight measurements can be made with confidence.
Purified to remove coloured contaminants that may interfere with spectrophotometric analysis. Zero free sulphates eliminate non-specific binding.
Tris(hydroxymethyl)aminomethane. Buffer salt used in both protein and DNA electrophoresis. Purified to remove ammonia and amine contaminants. Results in greater resolution during isoelectric focusing.
Catalogue Number Description Pack SizeEL0085 Tris Buffer (Base) 250gEL0086 Tris Buffer (Base) 1kg
A Non-ionic surfactant, used for Western blotting and other molecular biology and biochemistry applications. Tween 20. Specially purified to remove epoxidized contaminants.
Tween-20 is a Registered Trademark of ICI America