Clarit-E Modular Electroblotting

Mini, Mini Wide and Maxi Complete Electroblotting Systems

• Hinged cassettes
• 3 blot capacity
• High intensity electrodes
• Rapid set-up cooling

The Clarit-E Electroblotting Systems include both modules required for Slab Gel Electrophoresis and Electroblotting and accessories, to provide a complete Mini, Mini Wide or Maxi gel casting, running and electroblotting system. The electroblotting module includes three inter-locking cassettes and six fibre pads.

A high intensity current is generated by coiled platinum electrodes. These in conjunction with the advanced cooling features allow rapid electroblotting in as little as one hour.

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Clarit-E Electroblotting

• Ideal for Western blotting of proteins
• Open design, rigid cassettes for maximum current transfer

These stand-alone, complete units for electroblotting, Mini, Mini Wide and Maxi formats, include Clarit-E tank and lid, electroblotting insert, cassettes and fibre pads. Units are interchangeable with Clarit-E slab and tube gel inserts. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained.

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Accessories

Clarit-E Tank Sub Electroblotters.

• Ideal for wet electroblotting of proteins - Western blotting
• Up to five gel blot cassettes utilised at any one time
• Hinged cassettes for added convenience
• Accommodates gel thicknesses from 0.25 up to 3mm

Designed primarily for wet electroblotting of proteins, Tank Sub Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems with up to five gel blot cassettes utilised at any one time. This is especially useful in high throughput laboratories.

A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette’s open architecture ensures the maximum blot area allows direct transfer of current. Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent pH drifts in the buffer due to incomplete buffer mixing. Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy.

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Clarit-E Semi Dry Electroblotters

• Rapid transfer times
• Western, Southern and Northern Blots
• Economic Transfers due to very low buffer volumes
• Screw down lid - Gels from 0.25 up to 10mm thick can be blotted
• Uniform heat dispersion
• Long life electrodes

These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting - typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective Clarit-E vertical gel system. Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes – typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arching or damage.

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Clarit-E Capillary Blotting

• Permanent wick with optimized capillary action
• Efficient transfer of high and low molecular weight fragments
• Fast, easy set-up
• DNA transfer in 2 hours or less; RNA transfer in 3 hours or less
• Floating lid ensures even pressure without gel compression

These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized permanent wicks, which eliminate the requirement for overlapping wicks, offering greater consistency.

The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for faster transfer times.

Side by side transfer of multiple gels can be performed in a single set up.

Available in 15 x 26cm and 28 x 26cm formats.

Principle:

Through capillary action, the buffer travels from the reservoir to the absorbent towelling via the porous supports, permanent wick, gel and membrane. This action optimises the transfer of low and high Molecular Weight DNA/RNA from gel to membrane without mechanical damage to even the thinnest of gels.

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Clarit-E dot and slot blotters

• Low cost
• Simple construction
• Easy assembly
• Four sample configurations
• Alphanumeric sample identification

Four different sample number size and style of Hybridisation Manifold are offered:- two types of dot blotter and two types of slot blotter.

Typical applications include clone screening with DNA / RNA probes in Southern / Northern blots and immunological screening with antibodies in western blots.

The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lateral transfer of samples – smudging – by ensuring that a complete vacuum is formed. Six thumbscrews ensure even and tight sealing for fast sample suck down.

Dot blotters are available in 48 and 96 well versions and slot blotters in 24 and 48 well.

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