QuickFISH™ Rapid Pathogen ID Kits - Product Information
Based on PNA FISH (Peptide Nucleic Acid Fluorescence In Situ Hybridisation) technology, QuickFISH uses fluorescent-labelled probes to target species-specific rRNA sequences in a highly sensitive and specific Fluorescence in situ hybridisation assay.
There is no need for further amplification or cell lysis to isolate genetic material as the PNA probes hybridise to rRNA inside the bacteria, enabling whole cell analysis.
QuickFISH uses PNA probes with paired quencher molecules. During hybridisation the probe separates from the quencher and is free to bind target rRNA in the sample. On cooling, unbound probe will reform probe-quencher complexes.
This eliminates the wash step required with the original PNA FISH assay, aiding convenience and reducing turn around time.
A sample of the positive culture can be fixed, hybridised, mounted and read with a fluorescent microscope in under 30 mins with minimal hands on processing time.
QuickFISH assay kits should be used together with QuickFISH Fixation Kits (QFFIXBC1-50, QFFIXBC1-25) which contain fixation reagents and QuickFISH slides. Results should be read using the AdvanDx Microscope Filter (AC007).
A QuickFISH starter system containing microscope filters, slide station, mixing station and 10µl fixed volume pipette is also available (ST004).
QuickFISH assays are not suitable for use with blood culture tubes containing charcoal as an antimicrobial removal device.
Download the QuickFISH Procedure Guide
QuickFISH duplex assay for determination of S. aureus and Coagulase Negative Staphylococcus in positive blood cultures.
The Staphylococcus QuickFISH assay is a duplex assay using two fluorescent probes to identify Staphylococcus aureus and Coagulase Negative Staphylococcus (CNS) in positive blood cultures that are reported as Gram-Positive cocci in clusters (GPCC).
The green probe visualises S. aureus whilst the red probe detects CNS. This accurate, early identification enables clinicians to distinguish between genuine infection and blood culture contamination.
IVD CE Marked.
QuickFISH duplex assay for determination of Enterococcus in positive blood cultures.
The Enterococcus QuickFISH assay is a duplex assay using two fluorescent probes to identify Enterococci faecalis and other Enterococci (OE) such as E. faecium in positive blood cultures that are reported as Gram-Positive cocci in pairs and chains(GPCPC). The green probe visualises E faecalis whilst the red probe detects E. faecium and other Enterococci enabling clinicians to prescribe appropriate therapy.
IVD CE Marked.
QuickFISH multiplex assay for determination of Gram Negative Rods in positive blood cultures.
The Gram-Negative QuickFISH assay is a multiplex assay using three fluorescent probes to identify Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae in positive blood cultures that are reported as Gram-Negative Rods (GNR). The green probe visualises E. coli, the red probe detects P. aeruginosa and the amber probe K.pneumoniae, enabling clinicians to treat patients appropriately.
The kit can distinguish between these three species in 20 minutes, meaning that pathogen ID can be reported alongside results from Gram-Staining. This allows clinicians to rapidly ensure early, appropriate therapy for patients with Pseudomonas bacteraemia, while minimising the unnecessary use of aggressive, broad-spectrum antibiotics. QuickFISH: Gram negative rods has the potential to improve patient outcomes through directing appropriate therapy, while also permitting improved pharmacovigilance as part of a wider antimicrobial stewardship programme.
Conventional culture techniques can take 2-5 days to make a positive species identification. Unlike C.albicans and C.parapsilosis, many C.glabrata isolates are not sensitive to fluconazole: species identity is needed to make a decision on appropriate treatment.
Candida QuickFISH allows clinicians to rapidly ensure early, appropriate antifungal therapy, facilitating better patient outcomes and reducing antifungal drug costs.
The Candida QuickFISH assay is a multiplex assay using three fluorescent probes to identify Candida in positive blood cultures that are reported as Yeasts. The green probe visualises C. albicans, the red probe detects C. glabrata and the amber probe C. parapsilosis enabling clinicians to treat patients appropriately.
The QuickFISH method requires minimal instrumentation and therefore is easy and inexpensive to implement. The starter system includes:
Dual Band Filter Set & Holder (AC007)
Slide Station (AC028)
Mixing Station (AC030)
10µl Pipette (AC029)
Individual components can also be supplied separately.
QuickFISH™ Kits and Accessories