Flow Cytometry

The range of sample preservation products include:

Streck Cell Preservative®

As immunophenotyping moved from the research laboratory in to the clinical laboratory, the need for controls grew as did the pressure on the instrument availability. Streck Cell Preservative®, which preserves whole blood, as well as many other sample types for up to seven days facilitates easy transport of samples and batching.

Cyto-Chex ® BCT

However, development did not stop there, and Cyto-Chex ® BCT was introduced, a direct-draw vacuum blood collection tube that contains a preservative reagent. The integrity of white blood cells is maintained at room temperature for three times longer than traditional methods.

CD-Chex® & CD-Chex® Plus

Developed over 15 years, CD-Chex® Plus controls for immunophenotyping by flow cytometry. They closely resemble patient samples with the inclusion of both red and white cells stabilised in a way which preserves the antigen sites. CD-Chex® also offer a complete process control when evaluating CD-34

 

Streck Cell Preservative

Cyto-Chex® stabilises cell morphologyStreck Cell Preservative is an easy-to-use liquid preservative that stabilises cell morphology and preserves cellular antigen expression.

  • Data available to support use with peripheral blood lumph nodes, bone marrow and cord blood
  • Samples collected in Streck Cell Preservative are stable for up to 7 days
  • Closed-Vial-Stability: 1 year at room temperature
  • Facilitates sample batching to reduce daily flow cytometry start-up costs
  • Simple 1:1 dilution with sample

The proof of Stability

Light scatter profiles comparing K2EDTA bone marrow samples diluted 1:1 with Streck Cell Preservative at six hours to the same K2EDTA sample diluted 1:1 with Streck Cell preservative analysed at three days post collection.

 

Light scatter profiles

Streck Cell Preservative

Streck Cell Preservative is a liquid preservative for:

  • bone marrow aspirates
  • surgical tissue samples
  • fine needle aspirates
  • peripheral blood
Streck Cell Preservative®
Product Code Description Pack Size Price Add to Cart
213350 Streck Cell Preservative 6 x 1ml £49.96   
213352 Streck Cell Preservative 24 x 1ml £186.60   
213355 Streck Cell Preservative 50 x 1ml £341.12   

 

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Cyto-Chex ® BCT

A vacuum tube for the collection, transport and retention of whole blood samples for immunophenotyping by flow cytometry.

  • Cell morphology and cellular antigen expression are maintained
    for up to 7 days
  • Stabilises white blood cell panel antigens including
    those of the HIV panel of CD markers
  • Reliable and accurate CD4 counts assured for up to
    7 days
  • Subsets of leucocytes can be distinguished by flow
    cytometry
  • Facilitates sample batching to reduce daily flow
    cytometry start-up costs

The Proof of Stability

Light scatter profiles from a blood sample drawn onto a K2EDTA tube analysed at six hours
compared to data from a blood sample from the same patient drawn into a Cyto-Chex ® BCT tube analysed at seven days.

 

BCT light scatter profiles

Cyto-Chex ® BCT
Product Code Description Pack Size Price Add to Cart
213361 Cyto-Chex BCT (5ml) 1 x 6 vials £64.61   
213362 Cyto-Chex BCT (5ml) 1 x 25 vials £257.18   
213386 Cyto-Chex BCT (5ml) 1 x 100 vials £999.31   

 

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CD-Chex® Plus

Introduction:

Normal human peripheral bloodCD-Chex® Plus is a complete positive procedure control manufactured form normal human peripheral blood leukcocytes and erythrocytes.

The range has been extended to match the changing needs of laboratories and now includes:

  • CD-Chex® Plus - Screw-Cap Vials
  • CD-Chex® Plus - Cap-Pierceable Vials
  • CD-Chex® Plus BC

Each range includes CD4 Low control which offers decreased levels of CD4 positive cells (~ 200 cells/µl)

  • 30-day open-vial stability and 90-day closed-vial stability
  • Free participation in STATS®
Benefits of CD-Chex®

CD-Chex Plus determines the accuracy and reproducibility
of the immunophenotyping precedure including:

  • Satisfies both CAP and CDC quality control requirements
  • Provides two levels of CD4+ T-cells
  • Verification of mAb reagents
  • Validation of instrument performance
  • Validation of operator technique
  • Verification of red blood cell lysis

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CD-Chex® Plus - Screw-Cap Vials

CD-Chex® Plus is supplied in a format that can be used on any platform.

  • Assayed for 26 of the most common lymphoid and myeloid CD markers
CD-Chex® Plus Screw-Cap Vials
Product Code Description Pack Size Price Add to Cart
213325 CD-Chex Plus 1 x 2.5ml £86.56   
213326 CD-Chex Plus 2 x 2.5ml £160.58   
213327 CD-Chex Plus 5 x 2.5ml £388.91   
213330 CD4 Low 1 x 2.5ml £104.13   
213331 CD4 Low 2 x 2.5ml £191.10   
213332 CD4 Low 5 x 2.5ml £401.46   
213340 CD-Chex Plus, CD4 Low 2 x 2.5ml £160.56   
213341 CD-Chex Plus, CD4 Low 4 x 2.5ml £292.90   
213344 CD-Chex Plus, CD4 Low 10 x 2.5ml £608.45   

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CD-Chex® Plus - Cap-Cap Vials

CD-Chex Plus in a new plastic vial with pierceable caps.

  • Compatible with BD FACS™ Sample Prep Assistant
  • Assayed for 26 of the most common lymphoid and myeloid CD markers
CD-Chex® Plus Cap-Pierceable Vials - Click to view ordering information
Product Code Description Pack Size Price Add to Cart
213365 CD-Chex Plus 2 x 3ml £173.73   
213367 CD-Chex Plus 5 x 3ml £395.18   
213366 CD4 Low 2 x 3ml £202.09   
213368 CD-Chex Plus, CD4 Low 4 x 3ml £425.28   
213370 CD-Chex Plus, CD4 Low 10 x 3ml £657.87   

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CD-Chex® Plus BC

Optimised for Beckman Coulter flow cytometers

  • Compatible with Coulter PrepPlus automated sample processing instruments
  • Assayed for 22 of the most common lymphoid and myeloid CD markers
CD-Chex® Plus BC
Product Code Description Pack Size Price Add to Cart
213373 CD-Chex Plus BC 1 x 3ml £100.17   
213374 CD-Chex Plus BC 2 x 3ml £190.19   
213371 CD4 Low 1 x 3ml £114.60   
213372 CD4 Low 2 x 3ml £213.40   
213375 CD-Chex Plus BC & CD4 Low 4 x 3ml £357.55   

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CD-Chex® CD34CD-Chex CD34

A whole blood control for enumerating CD34 positive cells by flow cytometry.

  • 30-day open-vial stability and 90-day closed-vial
    stability
  • Compatible with both ISHAGE, Stem-kit and
    ProCOUNT
  • Represents samples containing CD34 positive cells
    having characteristics similar to progenitor cells
  • The product can be used with a variety of
    systems reagents and Class III antibodies

 

Typical Assay Value Ranges:
Level 1: 0.05% (3-5 cells/µl)
Level 2: 0.5% (30-50 cells/µl)
Level 3: 1.75% (90-150 cells/µl)
CD-Chex CD34
Product Code Description Level Pack Size Price Add to Cart
213336 CD-Chex CD34 1 1 x 1ml £106.64   
213337 CD-Chex CD34 1 2 x 1ml £204.49   
213346 CD-Chex CD34 2 1 x 1ml £106.64   
213347 CD-Chex CD34 2 2 x 1ml £204.49   
213382 CD-Chex CD34 3 1 x 1ml £160.58   
213383 CD-Chex CD34 3 2 x 2ml £294.82   
213338 CD-Chex CD34 1 & 2 2 x 1ml £206.67   
213339 CD-Chex CD34 1 & 2 4 x 1ml £375.11   

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Retic-Chex® II

Retic-Chex IIA whole blood control, manufactured with reticulocytes that resemble the appearence of patient samples for accurate identifications. Available in three levels which are well positioned to monitor the haemostatic response.

  • 14-day open-vial stability and 75-day closed-vial stability
  • Available in glass vials for automated methods
  • Retic-Chex Stain also available
  • Free participation in STATS®
Product Select Guide:
Manufacturer Instrument Compatible
BD Biosciences FACS Series v

 

Retic-Chex II
Product Code Description Pack Size Price Add to Cart
285506 Retic-Chex II Tri-Level 6 x 1ml £115.72   
285507 Retic-Chex II Level 1 2 x 1ml    
285510 Retic-Chex II Level 1 4 x 1ml £85.54   
285508 Retic-Chex II Level 2 2 x 1ml    
285511 Retic-Chex II Level 2 4 x 1ml £85.54   
285509 Retic-Chex II Level 3 2 x 1ml    
285512 Retic-Chex II Level 3 4 x 1ml £85.54   

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