Faecal Testing - Calprotectin

Calprotectin Assays Overview

The symptoms of organic inflammatory intestinal disease (IBD) and those of functional disorders such as Irritable Bowel Syndrome (IBS) can be very similar in presentation. Historically, clinical gastroenterologists have had to use invasive endoscopy to differentially diagnose between these conditions. Not only is this stressful for the patient, it is costly for the NHS.

Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with IBD, patients suffering from IBS do not have increased Calprotectin levels. Such increased levels are shown to correlate well with both endoscopic and histological assessment of disease activity.

The NHS Centre for Evidence-based Purchasing has conducted several reviews on calprotectin testing and its use in differentiating IBS and IBD. These reports conclude that using calprotectin assays supports improvements in patient management and offers substantial cost savings.

 

Measurement of Faecal Calprotectin – Your Choice

Alpha Laboratories offers the widest range of test formats for the measurement of human faecal Calprotectin.  Whether you want to perform the test within primary care, in the Gastroenterology clinic or the hospital laboratory, we have the right test to best suit your needs.

Alpha can also offer an automated solution to laboratories with a higher throughput of tests, with the availability of the Dynex DS2™ ELISA Processing System.

Clinical Applications

• Differential diagnosis: The Calprotectin assay offers an excellent negative predictive value enabling non-invasive differentiation between organic IBD and functional IBS.
• Therapy monitoring: Ensure compliance and successful therapy by detecting a decrease in calprotectin levels.
• Relapse prediction: An increase in calprotectin can be an indicator for possible IBD relapse in the future

Download a summary of some of the Clinical Publications referencing the Buhlmann Calprotectin Assays

Review the Literature

View this video to find out more about Calprotectin testing and the Buhlmann kits:

 

Calprotectin ELISA

An ELISA kit intended for the quantitative in vitrodetermination of human calprotectin in stool samples.

Features & Benefits:

• Easy-to-use ELISA kit (total assay time is 75 minutes)
• All reagents are ready to use*
• Kit includes calibrators and controls
• Analytical Sensitivity: <10µg/g Calprotectin in faecal sample
• Established cut-off of 50µg/g
• Measurable range 30-1800µg/g or 10-600µg/g**

* except wash buffer

** depending on which protocol is used

Product Code	Description	Pack Size	Price	Add to cart
EK-CAL	Calprotectin ELISA	96 wells	£586.50

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Quantum Blue - Quantitative Rapid Test

A rapid quantitative Calprotectin testing platform for lower throughput requirements.

If your Calprotectin test request number is too low to achieve a reasonable turn around time using an ELISA method, Quantum Blue provides a comprehensive, low cost, entry-level Calprotectin testing platform. Quantum Blue can be used in either the clinic or laboratory setting.

Features & Benefits

• Based on established lateral flow technology
• Provides a quantitative measure of Calprotectin in faecal samples but in a rapid test format
• Simple and rapid extraction and test process
• Supplied with a dedicated reader
• Excellent correlation with the market leading Calprotectin ELISA: uses a consistent cut off value (50µg/g)
• Measurable range 30-300µg/g or 100-1800µg/g Calprotectin in stool samples*

 

 

* depending on protocol used

 

 

Quantum Blue - Cick to view ordering information

Product Code.	Description	Pack Size	Price	Add to cart
LF-CAL25	Quantum Blue: Calprotectin tests (25)	25 tests	£440.64
LF-CHR25	Quantum Blue: Calprotectin tests - Extended Range (25)	25 tests	£440.64
BI-POCTR-ABS	Quantum Blue Reader	1 Unit	£2,971.91

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CalDetect - Semi quantitative rapid test

A rapid, semi-quantitative Calprotectin test that can be used either in the primary care or clinic setting.

CalDetect is a stand alone test and does not require laboratory infrastructure, additional devices or instruments.

Features & benefits

• Fast & simple point-of-care test
• Results obtained in 10 minutes
• Kit includes faecal collection paper, extraction tube and lateral flow test cassette
• CE marked for home use

The performance characteristics of CalDetect have been proven similar to that of an established ELISA²

 

Reference: 2. Shastri Y et al. (2006) A prospective comparative study for new rapid bedside faecal Calprotectin test with an established ELISA to assess intestinal inflammation.  107^th Annual Meeting of AGA: Abstract No. S1314

 

PreventID CalDetect - click to view ordering information

Product Code	Description	Pack Size	Price	Add to Cart
KST11005	PreventID CalDetect	10 tests	£157.08

 

Handling Stool Samples

A range of products are now available to simplify and assist the pre-analytical faecal extraction process. These are dedicated, specially designed products to minimise operator contact with the sample whilst optimising extraction of the analytical parameter.

Product Code	Description	Pack Size	Price
B-CAL-RD	Smart-Prep Faecal Sample Preparation Kit (For use with Calprotectin ELISA & Quantum Blue)	50 Tubes	£159.12
B-CAL-SO50	ScheBo® Quick-Prep XL Faecal Sample Preparation Kit (For use with Calprotectin ELISA & Quantum Blue)	50 Tubes	£159.12

 

 

 

 

 

 

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Find Out More About The Calprotectin Range of products

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