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A1c-Cellular™ Whole Blood Control
Ammonia Ultra
Amylase Liquid
Angiotensin Converting Enzyme (ACE)
Bile Acids
Bilirubin (Vanadate method)
Calprotectin ELISA
Calprotectin Rapid Test (CalDetect)
Calprotectin Rapid Test (Quantum Blue)
Cellular Allergen Stimulation Test (CAST) System
CH50-Total Complement Activity, Auto-Kit
Cholesterol - High Density Lipoprotein (HDL)
Cholesterol - Low Density Lipoprotein (LDL)
C-Reactive Protein Extended Range (erCRP) Reagent
C-Reactive Protein - High sensitivity (CRP-HS) Reagent
Haptoglobin
3 - hydroxybutyrate
Immunoglobulins
Ketone Bodies, Total
Lipoprotein (a)
Non-Esterified Fatty Acids (NEFA-HR)
Rheumatoid Factor
Triglycerides
Zinc
A whole blood control which tests the entire HbAIc procedure, including lysing of the red blood cells
A1c-Cellular™ offers:
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Ammonia, derived from the catabolism of amino acids and from the action of intestinal bacteria on dietary protein, is converted to urea in the liver hepatocytes and so rendered non toxic. Studies have shown that excess ammonia can have a toxic effect on the central nervous system and clinical manifestations are typically neurological disturbances. Elevated ammonia may also be observed in severe liver failure as may occur in Reye’s Syndrome, viral hepatitis or cirrhosis.
This kit is based on an enzymatic UV method (GLDH).
Alpha-amylases are hydrolytic enzymes which break down starch into maltose. In the human body alpha-amylases originate from various organs. As diseases of pancreas exhibit few specific clinical symptoms, alpha-amylase assays are important in the field of pancreas diagnostics. Besides occurring in chronic pancreatitis and inflammatory phase of acute pancreatitis, hyperamylasemia can also be found in renal failure, in pulmonary and salivary glands disease and in cerebral trauma, surgical operations and macroamylasemia. To confirm pancreatic-specificity, it is always recommended that another pancreas specific enzyme be determined, such as lipase or pancreatic alpha-amylase.
This kit is based on a kinetic protected pNPG7 (EPS) method.
For the determination of ACE activity in serum.
ACE activity is used as a predictive test for diseases such as sarcoidosis and hypertension. Elevated ACE activity is a risk factor for myocardial infarction.
Open-Vial Stability:
Hydrolysis of FAPGG results in a decrease in absorbance and is directly proportional to the ACE activity in the sample.
Expected Values:
Normal Adults (aged 20-70): 12-68 ACE UnitsNormal Children (aged 0.5-19yrs): 27-133 ACE Units
Performance Date:
Sensitivity - Minimum detectable dose of ACE: 12 ACE Units
Linearity - Kinetic method is linear from 12-150 ACE Units
Precision - Determined by measuring three different serum samples 20 times in a single run
Intra-assay precision: 2.7%Inter-assay precision: 8.1%
References:
For the determination of total bile acids in serum or plasma.
Raised levels in pregnancy are indicative of cholestasis. An increased level in fasting or postprandial state is considered a specific indicator of liver disease. Decreased levels indicate bile acid malabsorption.
bile acids + Thio-NAD Oxid. bile acids + Thio-NADH
Oxid.bile acids + NADH bile acids + NAD
3-a Hydroxysteroid dehydrogenase (3-a HSD) oxidizes bile acids to 3 keto steroids, whereby Thio-NAD is reduced to Thio-NADH. The reaction is reversible and the same enzyme can convert 3-keto steroids and NADH to bile acids and NAD (recycling of bile acids). The rate of formation of the Thio-NADH is determined by measuring change of absorbance at 405 nm.
Expected Values: 0-10µmol/L in serum or plasmaSensitivity: Lower limit of linearity is 1µmol/L
Serum bilirubin measurement is widely used as a screening test for liver functions. Total bilirubin is the sum of the unconjugated and conjugated fractions, and is elevated in conditions causing obstruction of the bile duct, hepatitis, cirrhosis, in haemolytic disorders and several inherited enzyme deficiencies. Indirect bilirubin is elevated by pre-hepatic causes such as haemolytic disorders or liver diseases resulting in impaired entry, transport or conjugation within the liver.
The vanadate oxidation method for measuring serum bilirubin shows a good correlation with the Acid Diazo method but has advantages in terms of low signal interference by co-existent serum substances and an extended stability in liquid form
The CAST-2000âELISA assay and Flow2 CASTâbasophil activation test represent a unique combination of methods for allergy testing. These tests are used in conjunction with purified allergens to elicit the response in vivo from patient blood samples.
The Flow2 CAST flow-cytometry based assay determines the in vitro activation of patient-derived basophils after incubation with specific allergens. The activation marker for this assay is CD63, whose surface expression is enhanced on the surface of basophils after degranulation in response to allergens. The kit allows both the allergen stimulation phase and the CD63 analysis to be performed on a whole blood sample. The new method involves direct identification of basophils in a whole blood sample by a combination of fluorescence labelling of a basophil marker (CCR3) and cell morphology. CD63 analysis on a whole blood sample eliminates the task of performing a time-consuming leukocyte isolation step. These new features of the Flow2 CAST test allow a result to be determined in less than one hour.
In the CAST-2000 ELISA, isolated, sedimented leukocytes from patient blood are simultaneously primed with Interleukin 3 (IL-3) and stimulated with allergens. Basophilic cells among others generate the allergic mediator, sulfidoleukotriene LTC4, and its metabolites LTD4and LTE4. These freshly synthesized sLT are subsequently measured in an ELISA test (Enzyme Linked ImmunoSorbent Assay) as a measure of the patient’s reaction to the allergen. The principle of the CAST-ELISA is covered by a patented technology (Patent No. US5487977).
For the determination of total complement activity in serum.
Complement activity is a direct indicator of abnormalities of the complement system. It has been correlated with the active stage of systemic lupus erythematosus (SLE), rheumatoid arthritis and inherited deficiences of the complement system2
2. Schur PH, Complement studies of sera and other biologic fluid. Hum Pathol 1983; 14: 338-42
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For the determination of HDL cholesterol in serum.
There is a strong negative relationship between the concentration of HDL cholesterol and coronary heart disease (CHD). 3
HDL cholesterol is used as a predictive test for patients at risk of developing CHD.
This reagent is intrinsically less susceptible to interference from icteric, ipaemic and haemolysed samples than reagents which use a detergentto remove LDL, VLDL & Chylomicrons.
3. Rifai, N and Warnick, G.R., Ed. "Laboratory measurement of Lipids, Lipoproteins andApoplipoproteins" AACC Press, Washington, DC, USA, 1997
For the determination of LDL cholesterol in serum or plasma.
A strong positive relationship has been reported between LDL cholesterol concentrations and the incidence of CHD4.
LDL cholesterol is used as a predictive test for patients at risk of developing CHD.
4. Burtis, C.A. and Ashwood E.R., Ed. Tietz Textbook of Clinical Chemistry, 2nd Ed., Saunders, Philadelphia, (1994).
CRP is recognised as one of the most sensitive acute phase proteins and is elevated in many diseases with a component of active inflammation or tissue destruction.
The CRP reagent is based on a standard turbimetric immunoassay.
Serum: Up to 5.0mg/L (adults). Up to 10mg/L (neonates up to 3 days)
Sensitivity: 2mg/L with a broad dynamic range to 280mg/L which minimises sample dilution
This kit is formulated to have two measurable ranges depending on the calibrators used (see below).
For the determination of haptoglobin in serum.
Increased levels of Haptoglobin, a transport molecule for haemoglobin are associated with inflammation, collagenoses, coronary disorders, Hodgkin's disease, nephrotic syndrome and tuberculosis.
Decreased levels are found in haemolytic anaemia, liver disease, congenital deficienceis and acute malaria.
For the determination of 3-hydroxybutyrate in serum or plasma.
For the determination of immunoglobulin in serum and plasma.
The quantification of immunoglobulins in serum is important for diagnosis, monitoring and prognosisof chronic liver disease, infectious diseases, lymphocytosis, multiple myeloma and other diseases.
For the determination of total ketone bodies in serum or plasma.
Ketone bodies assays are used in diagnosis of diabetes, since the concentration in the blood increases in hyperlipolysis due to a disorder in sugar metabolism.
For the determination of lipoprotein (a) (Lp(a)) in serum or plasma.
The structure of Lp(a) was found to be quite similar to that of plasminogen. In consideration of the structural characteristics of Lp(a) it was proposed that Lp(a) was related to athersclerosis. Cholesterol is one of the diagnostic aids for the diagnosis of atherosclerosis. Lp(a) is considered another risk factor, which is independent of cholesterol.
For the determination of Non-Esterified Fatty Acids in serum.
Non-esterified fatty acids (NEFA) in serum are used as an important energy source for peripheral tissues. The amount of NEFA in serum depends on a balance between intake in liver and peripheral tissues and release from adipose tissues. The amount of NEFA decreases by physical exercise and increases by starvation, cold or smoking. Alterations in NEFA levels are also observed in diabetes, hepatic diseases or endocrine diseases.
Technical Information:
For the determination of rheumatoid factor (RF) activity in serum.
RF is elevated in the serum of rheumatoid arthritis patients and is a specific biomarker for this disease.
Triglycerides are esters of glycerol with three fatty acids. Measurement of triglycerides is used in screening of the lipid status to detect atherosclerotic risks. High triglyceride levels also occur in various diseases of liver, kidneys and pancreas.
The kit is based on an enzymatic colorimetric Trinder method.
Triglycerides are hydrolised by lipoproteinlipase (LPL) to glycerol and fatty acids; glycerol is phosphorilized to glycerol-3-phosphate in presence of glycerolkinase (GK) and adenosine 5-triphosphate (ATP) and then converted by glycerol-3-phosphate oxidase (GPO) into dihydroxyacetonephosphate and hydrogen peroxide. In the presence of peroxidase (POD), the hydrogen peroxide oxidizes the chromogen 4-aminoantipyrine and TOOS* to form a red compound which colour intensity is proportional to the concentration of analyte in the sample.
* N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline
For the determination of zinc in serum, plasma, cerebrospinal fluid and urine by colorimetric assay.
Zinc is a cofactor for numerous enzymes.
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